Hydrophilic interaction chromatography is a variation of normal phase chromatography in which a polar stationary phase is used with a mobile phase that contains a high concentration of water-miscible organic solvent and a low concentration of aqueous eluent. The main retention mechanism is the partitioning of the polar analytes between the polar stationary and the non-polar mobile phase. As it is also called "aqueous normal phase," the elution order is similar to that of normal phase and the sample elution is in the order of increasing hydrophilicity. Without using ion-pair reagents, COSMOSIL HILIC retains highly polar analytes that would not be retained in reversed phase chromatography. It also shows a weak anion-exchange mechanism with the positively charged stationary phase; thus acidic compounds are strongly retained.
- Triazole-bonded stationary phase
- Enhanced hydrophilic interaction
- Excellent retention for highly polar analytes
- Unique anion-exchange mechanism
Specifications:
| Packing Material | HILIC |
| Silica Gel | High purity porous spherical silica |
| Average Particle Size | 5 µm |
| USP Code | L104 |
| Specific Surface Area | approx. 300 m2/g |
| Interactions | Hydrophilic Interaction, Anion Exchange |
| Suitable Samples | Hydrophilic Compounds, Acidic Compounds |
| Features | Suitable for non-retaining by C18 |
