Nacalai Tesque COSMOSIL RNA-RP1 Analytical HPLC Column, 5 µm, 4.6 x 250 mm, 1/Pk - 21490-11

Cosmosil® RNA-RP1

Nacalai Tesque COSMOSIL RNA-RP1 Packed Analytical HPLC Column, 5μm, 4.6mm I.D. x 250mm Length, 1/Pk

Product Number: 21490-11

HPLC columns for RNA and Oligonucleotide Separation

RNA-based therapeutics represent a powerful new tool for gene therapy. In RNA production, impurities such as nucleotide triphosphates, abortive transcripts, and DNA templates can affect downstream applications by triggering immune responses. Gel-based methods of purification, such as preparative denaturing polyacrylamide gel electrophoresis (PAGE) and agarose gels, are limited by poor resolution of RNA size and potential chemical modifications from reagents such as formaldehyde. To date, high performance liquid chromatography (HPLC) remains a staple method for purification of messenger RNA (mRNA) and RNA oligonucleotides. Presented here are two HPLC methods: a size exclusion-based method for the purification of long mRNA, and a reversed-phase method for the purification of oligonucleotides.

The COSMOSIL RNA series is comprised of RNA-RP1, a reversed-phase column, and RNA-SEC-1000 and RNA-SEC-2000, size-exclusion columns.

• Detects impurities not observable with electrophoresis.
• Scale up to preparative separations.
• Separate long RNA strands from 100 to 5,000 bases.
• Two modes available: reversed-phase and size-exclusion.

Product Specifications:

Column Specifications:

Additional Column Sizes are available - Please contact our Sales Team with your requirements.

Cosmosil Columns Online Catalogue:

• Nacalai Tesque COSMOSIL HPLC Columns
• Nacalai Tesque COSMOCORE UHPLC Columns
• Nacalai Tesque Reagents, Standards, Solvents