PROTEIN KW / PROTEIN LW columns separation by size also for high molecular weight
PROTEIN KW-802.5 HPLC Standard Gel Filtration (GFC) Column - 5μm - 8.0mm I..D. x 300mm Length
Shodex Product Number: F6989000
The silica-based SEC (GFC) column for proteins and enzymes
Specifications:
| Description | PROTEIN KW-802.5 300x8mm (KW-800 Series) | 
|---|---|
| Chemistry Producers Term | KW | 
| Mode of Separation | Gel Filtration (GFC) / SEC Size Exclusion | 
| Plate Number (TP/column) | ≥ 21,000 | 
| Housing material | steel | 
| Maximum pressure | 5.0 MPa (50 bar) | 
| Usual flow rate | 1.0 mL/min | 
| Maximum flow rate | 1.5 mL/min | 
| Temperature range | 4 to 45°C | 
| pH range | 3.0 to 7.5 | 
| Salt concentration | ≤ 0.3 M | 
| Shipping solvent | H2O | 
| MW range | 5,000 - 100,000 | 
| Exclusion limit | 150,000 | 
| Base Material | Silica | 
| Functional Group, Ligand | Hydrophilic polymer | 
| Shipping Solvent | H2O | 
| Internal Diameter | 8 mm | 
| Length | 300 mm | 
| Brand | PROTEIN | 
| Particle Morphology | spherical, porous | 
| Particle Size | 5 µm | 
| Pore Size | 150 Å (max. 400 Å) | 
| USP Class | L20, L33, L59 | 
PROTEIN KW-800 series Features
The packing material of the Shodex PROTEIN KW-800 series is based on silica particles whose surface is coated with a hydrophilic polymer. This column is for aqueous SEC (Gel filtration chromatography (GFC)). It enjoys a positive reputation thanks to its high recovery ratio of proteins and peptide. The PROTEIN KW-800 series comprises three kinds of columns, differing in pore and particle size.
- Silica-based packed columns for aqueous SEC (GFC) analysis
 - Suitable for the analysis of proteins and enzymes
 - Fulfills USP-NF L20 - Dihydroxypropane groups chemically bonded to porous silica or hybrid particles, 1.5-10 μm in diameter, or a monolithic silica rod.
 - Fulfills USP-NF L33 - Packing having the capacity to separate dextrans by molecular size over a range of 4,000 to 500,000 Da. It is spherical, silica-based, and processed to provide pH stability.
 - Fulfills USP-NF L59 - Packing for the size-exclusion separations of proteins (separation by molecular weight) over the range of 5 to 7000 kDa. The packing is a spherical 1.5- to 10-μm, silica or hybrid packing with a hydrophilic coating.
 - Suitable for: Proteins Peptides - SEC Size Exclusion
 
PROTEIN KW-802.5 Possible Applications
- Effects of Flow Rate (KW-802.5, KW-803)
 - Recovery of Proteins (KW-802.5 and KW-803)
 - Standard Proteins (1) (KW-802.5)
 - Standard Proteins (14) (KW-802.5)
 - Standard Proteins (2) (KW-802.5 and KW-803)
 - Analysis of Enoxaparin Sodium (KW-803 + KW-802.5)
 - Analysis of Exenatide According to USP-NF Method (KW-802.5)
 - Analysis of High Molecular Impurities in Insulin Aspart According to Pharmacopeia Method (KW-802.5)
 - Analysis of Insulin According to USP-NF Method (KW-802.5)
 - Analysis of Insulin Glargine According to JP Method (KW-802.5)
 - Analysis of Insulin Glargine According to USP-NF Method (KW-802.5)
 - Analysis of Insulin Human According to JP Method (KW-802.5)
 - Analysis of Semaglutide (KW-802.5)
 - Analysis of Teriparatide According to EP Method (KW-802.5)
 - Analysis of Thymosin α1 (KW-802.5)
 - PEG-Chymotrypsin (KW-802.5)
 - SEC Analysis of Liraglutide Formulation (KW-802.5)
 - SEC Analysis of Low Molecular Weight Heparin (1) (Dalteparin Sodium) (KW-803 + KW-802.5)
 - SEC Analysis of Low Molecular Weight Heparin (2) (Parnaparin Sodium) (KW-803 + KW-802.5)
 - Comparison between KW402.5-4F and KW-802.5
 
Range of Aqueous SEC (GFC) Columns: Silica-based Columns
| Product Code | Product Name | Plate Number (TP/column)  | 
			Particle Size (μm)  | 
			Pore Size (Å)  | 
			Column Size (mm) I.D. x Length  | 
			Shipping Solvent | 
| KW-800 Series | Standard columns | Base Material: Silica | ||||||
| F6989000 | PROTEIN KW-802.5 | ≥ 21,000 | 5 | 400 | 8.0 x 300 | H2O | 
| F6989103 | PROTEIN KW-803 | ≥ 21,000 | 5 | 1,000 | 8.0 x 300 | H2O | 
| F6989104 | PROTEIN KW-804 | ≥ 16,000 | 7 | 1,500 | 8.0 x 300 | H2O | 
| F6700131 | PROTEIN KW-G 6B | (guard column) | 7 | — | 6.0 x 50 | H2O | 
| KW400 Series | High performance semi-micro columns | Base Material: Silica | ||||||
| F6989201 | KW402.5-4F | ≥ 35,000 | 3 | 400 | 4.6 x 300 | H2O | 
| F6989202 | KW403-4F | ≥ 35,000 | 3 | 800 | 4.6 x 300 | H2O | 
| F6989203 | KW404-4F | ≥ 25,000 | 5 | 1,500 | 4.6 x 300 | H2O | 
| F6989204 | KW405-4F | ≥ 25,000 | 5 | 2,000 | 4.6 x 300 | H2O | 
| F6700132 | KW400G-4A | (guard column) | 5 | — | 4.6 x 10 | H2O | 
| For antibody drugs analysis | Standard columns | Base Material: Silica | ||||||
| F6989303 | PROTEIN LW-803 | ≥ 12,000 | 3 | 1,000 | 8.0 x 300 | H2O | 
| F6700133 | PROTEIN LW-G 6B | (guard column) | 3 | — | 6.0 x 50 | H2O | 
| For antibody drugs analysis | Semi-micro columns | Base Material: Silica | ||||||
| F6989403 | PROTEIN LW-403 4D | ≥ 11,000 | 1.9 | 1,000 | 4.6 x 150 | H2O | 
| F6700134 | PROTEIN LS-G 4J | (guard column) | 1.9 | — | 4.6 x 20 | H2O | 
- Model: F6989000
 - Manufactured by: Showa Denko
 

					
											














