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Shodex HPLC Column PROTEIN KW405-4F ID 4,6mm L 300mm - F6989204

Price: £1,431.83

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PROTEIN KW / PROTEIN LW columns separation by size also for high molecular weight

PROTEIN KW405-4F HPLC High performance semi-micro column - Gel Filtration (GFC) - 5μm - 4.6mm I.D. x 300mm Length

Shodex Product Number: F6989204

The silica-based SEC (GFC) column for proteins and enzymes


Description PROTEIN KW405-4F 300x4.6mm
Chemistry Producers Term KW
Gel material Silica
Functional group Hydroxyl / Hydrophilic polymer
Mode of Separation Gel Filtration (GFC) / SEC Size Exclusion
Plates per column ≥ 25,000
Housing material steel
Maximum pressure 7.0 MPa (70 bar)
Usual flow rate 0.35 mL/min
Maximum flow rate 0.5 mL/min
Temperature range 5 to 45°C
pH range 3.0 to 7.5
Salt concentration ≤ 0.3 M
Shipping solvent H2O
MW range 200,000 - (20,000,000)
Exclusion limit (20,000,000)
Internal Diameter 4.6 mm
Length 300 mm
Particle Morphology spherical, porous
Particle Size 5 µm
Pore Size 1,000 Å (max. 2,000 Å)
USP Class L20, L33, L59

PROTEIN KW400 series

The Shodex KW400-4F series is the high-performance semi-micro column version of the KW-800 series. The theoretical plate number and the sensitivity are improved by reducing the diameter of the packed particle to 3 micrometers. KW405-4F, the newest addition to the KW400 line-up, has a larger pore size than any columns in the existing KW-800 series, and can analyze proteins whose molecular weight is larger than 1,000,000. It enables the scientist to carry out the analysis of protein complexes.

  • Reduced packing material particle size enhances column performance
  • Three to four-fold higher sensitivity than KW-800 series
  • KW405-4F is applicable analyzing samples with molecular weight above 1,000,000
  • Fulfills USP-NF L20 - Dihydroxypropane groups chemically bonded to porous silica or hybrid particles, 1.5-10 μm in diameter, or a monolithic silica rod.
  • Fulfills USP-NF L33 - Packing having the capacity to separate dextrans by molecular size over a range of 4,000 to 500,000 Da. It is spherical, silica-based, and processed to provide pH stability.
  • Fulfills USP-NF L59 - Packing for the size-exclusion separations of proteins (separation by molecular weight) over the range of 5 to 7000 kDa. The packing is a spherical 1.5- to 10-μm, silica or hybrid packing with a hydrophilic coating.
  • Suitable for: Proteins Peptides - SEC | Aqueous SEC (GFC) - Biological macromolecules
    (Proteins, Peptides, Nucleic acids, etc.) - Buffer etc.

PROTEIN KW405-4F Possible Applications

  • Standard Proteins (26) (KW404-4F)
  • Particle Size Distribution Determination of Hepatitis B Surface Antigen Virus-like Particle (HBsAg VLP) (KW405-4F)
  • Analysis of Hemocyanin (KW405-4F)
  • SEC Analysis of Latex Particles (KW405-4F)

Range of Aqueous SEC (GFC) Columns: Silica-based Columns

Product Code Product Name Plate Number
Particle Size
Pore Size
Column Size (mm)
I.D. x Length
Shipping Solvent
KW-800 Series | Standard columns | Base Material: Silica 
F6989000 PROTEIN KW-802.5 ≥ 21,000 5 400 8.0 x 300 H2O
F6989103 PROTEIN KW-803 ≥ 21,000 5 1,000 8.0 x 300 H2O
F6989104 PROTEIN KW-804 ≥ 16,000 7 1,500 8.0 x 300 H2O
F6700131 PROTEIN KW-G 6B (guard column) 7 6.0 x 50 H2O
KW400 Series | High performance semi-micro columns | Base Material: Silica
F6989201 KW402.5-4F ≥ 35,000 3 400 4.6 x 300 H2O
F6989202 KW403-4F ≥ 35,000 3 800 4.6 x 300 H2O
F6989203 KW404-4F ≥ 25,000 5 1,500 4.6 x 300 H2O
F6989204 KW405-4F ≥ 25,000 5 2,000 4.6 x 300 H2O
F6700132 KW400G-4A (guard column) 5 4.6 x 10 H2O
For antibody drugs analysis | Standard columns | Base Material: Silica
F6989303 PROTEIN LW-803 ≥ 12,000 3 1,000 8.0 x 300 H2O
F6700133 PROTEIN LW-G 6B (guard column) 3 6.0 x 50 H2O
For antibody drugs analysis | Semi-micro columns | Base Material: Silica
F6989403 PROTEIN LW-403 4D ≥ 11,000 1.9 1,000 4.6 x 150 H2O
F6700134 PROTEIN LS-G 4J (guard column) 1.9 4.6 x 20 H2O

  • Model: F6989204
  • Manufactured by: Showa Denko