HILICpak VG-50 columns with tertiary amino groups
HILICpak VG-50G 2A HPLC Semi-micro Guard Column - 5 μm - 2.0mm I.D. x 10mm Length
Shodex Product Number: F6711200
The robust Polymer-based Amino HILIC column for many general applications
Specifications:
Description | HILICpak VG-50G 2A 10x2.0mm |
---|---|
Quantity | 1 pc |
Chemistry Producers Term | VG |
Gel material | Polyvinyl alcohol |
Functional group | Amino |
Mode of Separation | Polymer-based Hydrophilic Interaction Chromatography HILIC |
Housing material | PEEK |
Usual flow rate | 0.1 to 0.2 mL/min |
Maximum flow rate | 0.45 mL/min |
Temperature range | 4 to 60°C |
pH range | 2 to 13 |
Shipping solvent | H2O/CH3CN = 15/85 |
Internal Diameter | 2.0 mm |
Length | 10 mm |
Brand | HILICpak |
Particle Size | 5 µm |
Pore Size | 100 Å |
VG-50 2D allows simultaneous analysis of saccharides, organic acids and amino acids with LC/MS detection under alkaline conditions. High anionic substances elute under alkaline conditions. Furthermore, alkaline conditions promote the deprotonation of hydroxyl groups at the time of ionization. Therefore, alkaline conditions are suitable for high sensitive detection of substances with hydroxyl groups such as saccharides under the negative mode.
- Suitable for saccharide analysis using HILIC mode
- Recovers reducing saccharides with high ratio (usage of tert. amine groups to avoid building of Schiff base)
- Polymer-based packing material provides excellent chemical stability and minimum deterioration
- over an extended time period
- pH stability: 2 to 13 (silanol free)
- Easily regenerated by washing in an alkaline solution
- Also suitable for evaporative light scattering detector (ELSD), corona charged aerosol detector (CAD), and mass spectrometry (LC-MS/MS)
- Adsorption of acidic substances on the amino functional group.
- Flush with solvents in the following sequence; water, 0.1 M NaOH (aq.), water, and the
eluent. - Suitable for: Amino acids - HILIC | Organic acids - HILIC | Mono-, di-saccharides,
and sugar alcohols Saccharides and sugar alcohols - HILIC | Amino sugars - HILIC | Water-soluble vitamins - HILIC
VG-50 Series - Base Material: Polyvinyl alcohol
Product Code | Product Name | Plate Number (TP/column) | Functional Group | Particle Size (μm) |
Pore Size (Å) |
Column Size (mm) I.D. x Length |
Shipping Solvent |
Standard columns (Housing Material: SUS) | |||||||
F7630200 | HILICpak VG-50 4D | ≥ 5,500 | Amino | 5 | 100 | 4.6 x 150 | H2O/CH3CN = 20/80 |
F7630100 | HILICpak VG-50 4E | ≥ 7,500 | Amino | 5 | 100 | 4.6 x 250 | H2O/CH3CN = 20/80 |
F6711100 | HILICpak VG-50G 4A | (guard column) | Amino | 5 | 100 | 4.6 x 10 | H2O/CH3CN = 20/80 |
Semi-micro columns (Housing Material: PEEK) | |||||||
F7630300 | HILICpak VG-50 2D | ≥ 3,500 | Amino | 5 | 100 | 2.0 x 150 | H2O/CH3CN = 15/85 |
F6711200 | HILICpak VG-50G 2A | (guard column) | Amino | 5 | 100 | 2.0 x 10 | H2O/CH3CN = 15/85 |
VT-50 Semi-micro columns (Housing Material: PEEK) - Base Material: Polyvinyl alcohol
Product Code | Product Name | Plate Number (TP/column) |
Functional Group | Particle Size (μm) |
Pore Size (Å) |
Column Size (mm) I.D. x Length |
Shipping Solvent |
F7630400 | HILICpak VT-50 2D | ≥ 4,500 | Quaternary ammonium |
5 | 100 | 2.0 x 150 | 25 mM HCOONH4 aq./ CH3CN = 15/85 |
F6711300 | HILICpak VT-50G 2A | (guard column) | Quaternary ammonium |
5 | 100 | 2.0 x 10 | 25 mM HCOONH4 aq./ CH3CN = 15/85 |
VC-50 Semi-micro columns (Housing Material: PEEK) - Base Material: Polyvinyl alcohol
Product Code | Product Name | Plate Number (TP/column) |
Functional Group | Particle Size (μm) |
Pore Size (Å) |
Column Size (mm) I.D. x Length |
Shipping Solvent |
F7630700 | HILICpak VC-50 2D | ≥ 3,500 | Carboxyl | 5 | 100 | 2.0 x 150 | H2O |
F6711600 | HILICpak VC-50G 2A | (guard column) | Carboxyl | 5 | 100 | 2.0 x 10 | H2O |
VN-50 - Base Material: Polyvinyl alcohol
Product Code | Product Name | Plate Number (TP/column) |
Functional Group | Particle Size (μm) |
Column Size (mm) I.D. x Length |
Shipping Solvent |
Standard columns (Housing Material: PEEK) | ||||||
F7630500 | HILICpak VN-50 4D | ≥ 10,000 | Diol | 5 | 4.6 x 150 | H2O/CH3CN = 25/75 |
F6711400 | HILICpak VN-50G 4A | (guard column) | Diol | 5 | 4.6 x 10 | H2O/CH3CN = 25/75 |
Semi-micro columns (Housing Material: PEEK) | ||||||
F7630600 | HILICpak VN-50 2D | ≥ 3,500 | Diol | 5 | 2.0 x 150 | H2O/CH3CN = 25/75 |
F6711500 | HILICpak VN-50G 2A | (guard column) | Diol | 5 | 2.0 x 10 | H2O/CH3CN = 25/75 |
Preparative columns (Housing Material: SUS) [ Preparative columns are made to order. ] | ||||||
F6830100 | HILICpak VN-50 10E | ≥ 11,000 | Diol | 5 | 10.0 x 250 | H2O/CH3CN = 25/75 |
F6711400 | HILICpak VN-50G 4A | (guard column) | Diol | 5 | 4.6 x 10 | H2O/CH3CN = 25/75 |
- Model: F6711200
- Manufactured by: Showa Denko