Shodex HPLC Column HILICpak VT-50 2D ID 2mm L 150mm - F7630400

HILICpak VT-50 column with quaternary ammonium groups

HILICpak VT-50 2D Semi-micro HPLC Column - 5μm - 2.0mm I.D. x 150mm Length

Shodex Product Number: F7630400

The robust HILIC column for anionic substances (especially phosphate compounds)

Specifications:

• Suitable for anionic substances (especially phosphate compounds) analysis using HILIC mode
• Use of some eluents add ion exchange mode
• Polymer-based packing material provides excellent chemical stability and minimum deterioration over an extended time period
• Suitable for LC/MS analysis
• Suitable for: Acidic sugars - Ion exchange | Drugs Metabolites - HILIC | Water-soluble vitamins - HILIC |  Hormones / Neurotransmitters - HILIC 

Possibe Application:

Glyphosate analysis with HILICpak VT-50 column

Organophosphate herbicides such as glyphosate and glufosinate are globally used non-selective weed killer, effective for most weeds. Most countries set regulations for organophosphate herbicides use due to concerns on their toxicity to humans. Therefore, monitoring their concentrations in environment and foods are required. Those compounds are highly hydrophilic, and thus HPLC is a preferred analysis method. However, they are hardly retained under reversed phase mode. To compromise the problem, methods using pre-column derivatization or addition of ion-pair reagent to the eluent are often applied.

In this application, HILICpak VT-50 2D, a polymer-based HILIC mode column, was used under alkaline condition. The results demonstrated the method’s feasibility for the simultaneous LC/MS/MS analysis of glyphosate, glyfosinate, and their metabolites, as well as ethephon, a plant growth regulator which has similar structure to glyphosate. The method proved to provide 1 ng/mL level quantification of the compounds. The method developed here provides a simple and highly sensitive analysis without the use of derivatization nor use of ion-pair reagent. The pH of the eluent used was about 9, thus the system needs to be alkaline tolerant. The use of alkaline eluent improves the peak shape (less tailing) of glyphosate than using an acidic condition. Note that these phosphorus containing pesticides have tendencies of forming metal coordination compounds, therefore select PEEK materials for the flow-line after the injector wherever it's possible.

VG-50 Series - Base Material: Polyvinyl alcohol

VT-50 Semi-micro columns (Housing Material: PEEK) - Base Material: Polyvinyl alcohol

VC-50 Semi-micro columns (Housing Material: PEEK) - Base Material: Polyvinyl alcohol

VN-50 - Base Material: Polyvinyl alcohol

?