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Shodex HPLC Column SUGAR SH-G ID 6,0mm L 50mm - F6700080

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SUGAR SH columns with sulfo (H+) groups

SUGAR SH-G HPLC Standard Guard Column for simultaneous analysis of saccharides and organic acids HPLC - 10μm - 6.0mm I.D. x 50mm Length

Shodex Product Number: F6700080

The ion exclusion chromatography column for sugars and organic acids

Related Products:

Specifications

Description SUGAR SH-G 50x6.0mm
Quantity 1 pc
Chemistry Producers Term SH
Gel material styrene divinylbenzene copolymer
Separation Ion Exclusion
Functional group Sulfo (H+)
Plates per column (guard)
Housing material steel
Temperature range 50 to 60 (≤ 95°C)
Shipping solvent H2O
Internal Diameter 6.0 mm
Length 50 mm
Brand Sugar
Particle Size 10 µm
USP L17, L22
  • Columns for simultaneous analysis of saccharides and organic acids
  • Separates neutral sugars in size exclusion mode and organic acids in ion exclusion mode
  • Different exclusion limits available
  • Suitable for the analysis of uronic and aldonic acids
  • Fulfills USP L17 - Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the hydrogen form, 6 to 12 μm in diameter.
  • Fulfills USP L22 - A cation-exchange resin made of porous polystyrene gel with sulfonic acid groups, 5-15 μm in diameter.
  • Also suitable for: Organic acids - Ion exclusion (+ Reversed phase) | Acidic sugars - Ion exclusion | Saccharides and origanic acids - Ion exclusion + SEC 

Possible Applications:

  • Aliphatic Alcohols (1) (SH1011, SH1821)
  • Aliphatic Alcohols (6) (SH1011)
  • Analysis of Related Compounds of Alanine and Aspartic Acid According to USP-NF Method (SH1011)
  • Calibration Curve for Saccharides and Organic Acids (SH1011)
  • Glycerin and Glyceric Acid Analysis (SH1011)
  • Rapid Analysis of Malto-oligosaccharides, Organic Acids, and Ethanol (SH1011 8C)
  • Saccharides and Organic Acids (1) (SH1011)
  • Saccharides and Organic Acids (2) (SH1011)
  • Soft Drink (SH1011)
  • Xylooligosaccharides (3) (SH1011)

Eluents:

The pure water used as the mobile phase must be deionized water that has been passed through a 0.45 μm membrane filter. Before use, it must also be degassed to prevent generating bubbles in the column and at its outlet, because all columns of this series are heated being used. Use of degassing device is recommended.

SH type
Although deionized water can be used alone as the mobile phase, adsorption of alkalis on the packing will change the chromatogram. Addition of sulfuric or phosphoric acid to the mobile phase is, therefore, recommended to prevent that change. max 0.05 M H2SO4 / H3PO4 / HClO4

Sugars are slow in dispersing. A higher degree of resolution can, therefore, be obtained by heating the column to as high a temperature as the mobile phase permits. In normal operation, however, the standard conditions of use in Table above should be observed.

The columns of the SH type are not suited for high-temperature separation of sucrose and raffinose because their packing acts as a decomposing catalyst on those sugars. In case, however, use of the SH type for separation of such sugars is unavoidable, the column temperature should be lowered to about 40 °C to prevent their decomposition.

For simultaneous analysis of saccharides and organic acids

Standard columns | Base Material: Styrene divinylbenzene copolymer

Product Code Product Name Plate Number
(TP/column)
Functional Group Exclusion Limit
(Pullulan)
Particle Size
(μm)
Column Size (mm)
I.D. x Length
Shipping Solvent
F6378100 SUGAR SH1011 ≥ 17,000 Sulfo 1,000 6 8.0 x 300 H2O
F6378101 SUGAR SH1821 ≥ 17,000 Sulfo 10,000 6 8.0 x 300 H2O
F6700080 SUGAR SH-G (guard column) Sulfo 10 6.0 x 50 H2O
F6378104 SUGAR SH1011 8C ≥ 5,000 Sulfo 1,000 6 8.0 x 100 H2O

For organic acids, cyanide ions and cyanogen chloride

Standard columns | Base Material: Styrene divinylbenzene copolymer

Product Code Product Name Plate Number
(TP/column)
Functional Group Particle Size
(μm)
Column Size (mm)
I.D. x Length
Shipping Solvent
F6378030 RSpak KC-811 ≥ 17,000 Sulfo 6 8.0 x 300 0.1 % H3PO4 aq.
F6378033 RSpak KC-811 6E ≥ 13,000 Sulfo 6 6.0 x 250 0.1 % H3PO4 aq.
F6700030 RSpak KC-G 6B (guard column) Sulfo 10 6.0 x 50 0.1 % H3PO4 aq.
F6700010 RSpak KC-G 8B (guard column) Sulfo 13 8.0 x 50 0.1 % H3PO4 aq.

Use KC-G 8B for samples with relatively high impurities and KC-G 6B for samples with relatively low impurities.


  • Model: F6700080
  • Manufactured by: Showa Denko