Shodex HPLC Column SUGAR SH1011 8C ID 8,0mm L 100mm - F6378104

SUGAR SH columns with sulfo (H+) groups

SUGAR SH1011 8C HPLC Standard Column for simultaneous analysis of saccharides and organic acids HPLC - 6μm - 8.0mm I.D. x 100mm Length

Shodex Product Number: F6378104

The ion exclusion chromatography column for sugars and organic acids

Related Products:

SUGAR SH-G Guard Cartridge Column - F6700080

Specifications

• Columns for simultaneous analysis of saccharides and organic acids
• Separates neutral sugars in size exclusion mode and organic acids in ion exclusion mode
• Different exclusion limits available
• Suitable for the analysis of uronic and aldonic acids
• Fulfills USP L17 - Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the hydrogen form, 6 to 12 μm in diameter.
• Fulfills USP L22 - A cation-exchange resin made of porous polystyrene gel with sulfonic acid groups, 5-15 μm in diameter.

Possible Applications:

• Rapid Analysis of Malto-oligosaccharides, Organic Acids, and Ethanol (SH1011 8C)

Eluents:

The pure water used as the mobile phase must be deionized water that has been passed through a 0.45 μm membrane filter. Before use, it must also be degassed to prevent generating bubbles in the column and at its outlet, because all columns of this series are heated being used. Use of degassing device is recommended.

SH type
Although deionized water can be used alone as the mobile phase, adsorption of alkalis on the packing will change the chromatogram. Addition of sulfuric or phosphoric acid to the mobile phase is, therefore, recommended to prevent that change. max 0.05 M H2SO4 / H3PO4 / HClO4

Sugars are slow in dispersing. A higher degree of resolution can, therefore, be obtained by heating the column to as high a temperature as the mobile phase permits. In normal operation, however, the standard conditions of use in Table above should be observed.

The columns of the SH type are not suited for high-temperature separation of sucrose and raffinose because their packing acts as a decomposing catalyst on those sugars. In case, however, use of the SH type for separation of such sugars is unavoidable, the column temperature should be lowered to about 40 °C to prevent their decomposition.

For simultaneous analysis of saccharides and organic acids

Standard columns | Base Material: Styrene divinylbenzene copolymer

For organic acids, cyanide ions and cyanogen chloride

Standard columns | Base Material: Styrene divinylbenzene copolymer

Use KC-G 8B for samples with relatively high impurities and KC-G 6B for samples with relatively low impurities.