HiLoad™ Superdex™ prep grade Columns and Superdex prep grade Lab Packs
- Exceptional resolution with good recovery
- Superdex 30 prep grade for polishing of peptides
- Superdex 75 prep grade for recombinant and native proteins up to approx. 70 000 D
- Superdex 200 prep grade separates MAbs from aggregates, dimers and contaminants
- Reproducibility and quick preparative separations according to size
- 1/16" fittings for convenient connections to ÄKTA™ design systems
HiLoad™ Superdex™ prep grade Columns and Superdex prep grade Lab Packs
Technical Information
HiLoad Superdex prepacked gel filtration columns.
TECHNICAL SPECIFICATIONS | |
Superdex prep grade | |
Fractionation range (Mr) | |
- Globular proteins | |
Superdex 30 prep grade | < 1 × 104 |
Superdex 75 prep grade | 3 × 103-7 × 104 |
Superdex 200 prep grade | 1 × 104-6 × 105 |
- Dextrans | |
Superdex 75 prep grade | 5 × 102-3 × 104 |
Superdex 200 prep grade | 1 × 103-1 × 105 |
Matrix | Spherical composite of cross-linked agarose and dextran |
Average particle size | 34 μm |
Chemical stability | Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, 70% ethanol, 1 M NaOH (for cleaning-in-place) |
pH stability | 3-12 (working and long term) |
1-14 (short term) | |
Storage | |
Superdex 30 prep grade | 0.2 M sodium acetate in 20% ethanol |
Superdex 75 prep grade | 0.2 M sodium acetate in 20% ethanol |
Superdex 200 prep grade | 20% ethanol |
Storage temperature | 4°C to 30°C |
Prepacked HiLoad Columns | |
Bed volume | HiLoad 16/600: 120 ml HiLoad 26/600: 320 ml |
Sample volume | HiLoad 16/600: < 5 ml HiLoad 26/600: < 13 ml |
Linear flow rate | 10-50 cm/h |
Max. pressure | |
Over the packed bed during operation: | 3 bar (42 psi, 0.3 MPa) |
Column hardware: | 5 bar (73 psi, 0.5 MPa) |
Theoretical plates | > 13 000 m-1 |
Fitting | 1/16" (valco) |
- Model: 28-9893-31
- Manufactured by: GE HealthCare