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HiLoad 16/600 Superdex 75 pg - 1 x 120 ml - 28-9893-33

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HiLoad™ Superdex™ prep grade Columns and Superdex prep grade Lab Packs

  • Exceptional resolution with good recovery
  • Superdex 30 prep grade for polishing of peptides
  • Superdex 75 prep grade for recombinant and native proteins up to approx. 70 000 D
  • Superdex 200 prep grade separates MAbs from aggregates, dimers and contaminants
  • Reproducibility and quick preparative separations according to size
  • 1/16" fittings for convenient connections to ÄKTA™ design systems

HiLoad™ Superdex™ prep grade Columns and Superdex prep grade Lab Packs

Technical Information


 
HiLoad Superdex prepacked gel filtration columns.

TECHNICAL SPECIFICATIONS
Superdex prep grade
Fractionation range (Mr)  
- Globular proteins  
Superdex 30 prep grade < 1 × 104 
Superdex 75 prep grade 3 × 103-7 × 104 
Superdex 200 prep grade 1 × 104-6 × 105 
- Dextrans 
Superdex 75 prep grade 5 × 102-3 × 104 
Superdex 200 prep grade 1 × 103-1 × 105 
Matrix Spherical composite of cross-linked
agarose and dextran 
Average particle size 34 μm 
Chemical stability Stable in all common buffers:
1 M acetic acid, 8 M urea,
6 M guanidine hydrochloride,
30% isopropanol, 70% ethanol,
1 M NaOH (for cleaning-in-place) 
pH stability 3-12 (working and long term) 
 1-14 (short term) 
Storage 
Superdex 30 prep grade 0.2 M sodium acetate in 20% ethanol 
Superdex 75 prep grade 0.2 M sodium acetate in 20% ethanol 
Superdex 200 prep grade 20% ethanol 
Storage temperature 4°C to 30°C 
Prepacked HiLoad Columns
Bed volume HiLoad 16/600: 120 ml
HiLoad 26/600: 320 ml 
Sample volume HiLoad 16/600: < 5 ml
HiLoad 26/600: < 13 ml 
Linear flow rate 10-50 cm/h 
Max. pressure  
Over the packed bed during operation: 
3 bar (42 psi, 0.3 MPa) 
Column hardware: 5 bar (73 psi, 0.5 MPa) 
Theoretical plates > 13 000 m-1 
Fitting 1/16" (valco) 


  • Model: 28-9893-33
  • Manufactured by: GE HealthCare

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