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Superdex 75 5/150 GL HPLC Columns - 28-9205-04

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Superdex™ Peptide Columns

  • High-resolution separation of peptides and other small biomolecules of 100-7000 molecular weight. The only product available in the industry that offers this fractionation range.
  • Steep selectivity curve, high pore volume, and novel cross-linked agarose/dextran matrix give exceptional performance.
  • Powerful alternative and complement to traditional reversed phase chromatography of small peptides.
  • Tricorn™ high-performance column (10/300 GL) expertly packed with Superdex™.
  • Precision columns (PC) for micropurification and analysis¾10-fold smaller column volume than Tricorn™ columns.
TECHNICAL SPECIFICATIONS
Superdex™ Peptide
Fractionation range (Mr100-7000 
Matrix  Spherical composite of cross-linked agarose and dextran 
Average particle size 13 μm 
Chemical stability Stable in all common buffers:
1 M acetic acid, 8 M urea,
6 M guanidine hydrochloride,
30% isopropanol, 70% ethanol,
1 M NaOH (for cleaning-in-place) 
pH stability 1-14 (working, long and short term) 
Prepacked Superdex™ Peptide Columns*
Bed volume  
PC 3.2/30 2.4 ml 
Tricorn 10/300 GL 24 ml 
Sample volume  
PC 3.2/30 2-25 μl 
Tricorn 10/300 GL 25-250 μl 
Max. pressure  
PC 3.2/30 20 bar (290 psi, 2 MPa) 
Tricorn 10/300 GL 15 bar (220 psi, 1.5 MPa) 
Max. flow rate (H2O at 25°C) 
PC 3.2/30 150 μl/min 
Tricorn 10/300 GL 1.2 ml/min 
Theoretical plates > 30 000 m-1 
Storage 20% ethanol 
Storage temperature 4°C to 30°C 
* Columns are not suitable for use with ÄKTAprime™ plus system.

  • Model: 28-9205-04
  • Manufactured by: GE HealthCare