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Superdex 75 PC 3.2/30
- High-resolution separations of proteins, peptides, and other biomolecules according to size.
- Ideal for the polishing step in a purification procedure.
- Separations in two ranges from Mr 3000 up to 600 000 (globular proteins).
- Easy and predictable scale-up.
- Excellent reproducibility and durability.
- Tricorn™ High Performance Columns (10/300 GL) expertly packed with Superdex™.
Superdex 75 PC 3.2/30
Technical Information
| TECHNICAL SPECIFICATIONS | |
| Superdex™ | |
| Exclusion limit (Mr) | |
| Superdex™ 75 | 1 × 105 globular protein |
| Separation range (Mr) | |
| Superdex™ 75 | 3000-70 000 globular protein |
| Matrix | Spherical composite of cross-linked agarose and dextran |
| Average particle size | 13 μm |
| Chemical stability | Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, 70% ethanol, 1 M NaOH (for cleaning in place) |
| pH stability | 3-12 (working and long term), 1-14 (short term) |
| Superdex™ 10/300 GL Columns (Tricorn) | |
| Bed dimensions | 10 × 300 mm |
| Recommended sample volume | 25-250 μl |
| Bed volume | 24 ml |
| Max. pressure | |
| Superdex™ 75 | 18 bar (261 psi, 1.8 MPa) |
| Max. flow rate (H2O at 25°C) | |
| Superdex™ 75 | 1.5 ml/min |
| Theoretical plates | > 30 000 m-1 |
| Superdex™ PC 3.2/30 Columns* | |
| Bed dimensions | 3.2 × 300 mm |
| Bed volume | 2.4 ml |
| Recommended sample volume | 2-25 μl |
| Max. pressure | |
| Superdex™ 75 | 24 bar (348 psi, 2.4 MPa) |
| Max. flow rate (H2O at 25°C) | |
| Superdex™ 75 | 0.100 ml/min |
| Theoretical Plates | > 30 000 m-1 |
| * Optimized for SMART System | |
Separation of standard proteins on Tricorn™ Superdex™ 200.
Separation of monomer and dimer on Tricorn™ Superdex™ 75.
- Model: 17-0771-01
- Manufactured by: GE HealthCare
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