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Superdex Peptide PC 3.2/30 HPLC Columns - 17-1458-01

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Superdex Peptide PC 3.2/30

  • High-resolution separation of peptides and other small biomolecules of 100-7000 molecular weight. The only product available in the industry that offers this fractionation range.
  • Steep selectivity curve, high pore volume, and unique cross-linked agarose/dextran matrix give exceptional performance.
  • Powerful alternative and complement to traditional reversed phase chromatography of small peptides.
  • Tricorn™ high-performance column (10/300 GL) expertly packed with Superdex™.

PC columns are optimized for use with SMART System. PC columns require the Precision Column Holder or Column Holder 10 cm when used with chromatography systems other than SMART System.
 
Superdex Peptide, 75 and 200 prepacked in Tricorn columns offer unmatched high-performance gel filtration.

TECHNICAL SPECIFICATIONS
Superdex™ Peptide
Fractionation range (Mr) 100-7000 
Matrix  Spherical composite of cross-linked agarose and dextran 
Average particle size 13 μm 
Chemical stability Stable in all common buffers:
1 M acetic acid, 8 M urea,
6 M guanidine hydrochloride,
30% isopropanol, 70% ethanol,
1 M NaOH (for cleaning-in-place) 
pH stability 1-14 (working, long and short term) 
Prepacked Superdex™ Peptide Columns
Bed volume  
PC 3.2/30* 2.4 ml 
Sample volume  
PC 3.2/30* 2-25 μl 
Max. pressure  
PC 3.2/30* 20 bar (290 psi, 2 MPa) 
Max. flow rate (H2O at 25°C) 
PC 3.2/30* 150 μl/min 
Theoretical plates > 30 000 m-1 
*   PC columns are optimized for use with SMART System.

Separation of standard peptides on Superdex™ Peptide 10/300 GL

The separation of SPI peptides C0070 illustrates the difference in resolution for small peptides.The relative pore volume of Superdex™ Peptide is 55.5% compared to 39.1% for TSK G2000 SW.

Superdex Peptide PC 3.2/30

Technical Information

 
Reference

  1. Fägerstam, L. et al. Detection and characterisation of oligosaccharides in column effluents using surface plasmon resonance.Fourteenth International Symposium on the Separation and Analysis of Proteins, Peptides, and Polynucleotides, Heidelberg, Germany (1994).

  • Model: 17-1458-01
  • Manufactured by: GE HealthCare

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