Superdex™ Peptide Columns
- High-resolution separation of peptides and other small biomolecules of 100-7000 molecular weight. The only product available in the industry that offers this fractionation range.
- Steep selectivity curve, high pore volume, and novel cross-linked agarose/dextran matrix give exceptional performance.
- Powerful alternative and complement to traditional reversed phase chromatography of small peptides.
- Tricorn™ high-performance column (10/300 GL) expertly packed with Superdex™.
- Precision columns (PC) for micropurification and analysis¾10-fold smaller column volume than Tricorn™ columns.
TECHNICAL SPECIFICATIONS | |
Superdex™ Peptide | |
Fractionation range (Mr) | 100-7000 |
Matrix | Spherical composite of cross-linked agarose and dextran |
Average particle size | 13 μm |
Chemical stability | Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol, 70% ethanol, 1 M NaOH (for cleaning-in-place) |
pH stability | 1-14 (working, long and short term) |
Prepacked Superdex™ Peptide Columns* | |
Bed volume | |
PC 3.2/30 | 2.4 ml |
Tricorn 10/300 GL | 24 ml |
Sample volume | |
PC 3.2/30 | 2-25 μl |
Tricorn 10/300 GL | 25-250 μl |
Max. pressure | |
PC 3.2/30 | 20 bar (290 psi, 2 MPa) |
Tricorn 10/300 GL | 15 bar (220 psi, 1.5 MPa) |
Max. flow rate (H2O at 25°C) | |
PC 3.2/30 | 150 μl/min |
Tricorn 10/300 GL | 1.2 ml/min |
Theoretical plates | > 30 000 m-1 |
Storage | 20% ethanol |
Storage temperature | 4°C to 30°C |
* Columns are not suitable for use with ÄKTAprime™ plus system. |
- Model: 17-5176-01
- Manufactured by: GE HealthCare