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Tosoh TSKgel Boronate-5PW Column, 10μm, ID 7.5 mm x L 7.5 - 13066

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Affinity Chromatography

 
TSKgel Boronate-5PW Column, 10μm, ID 7.5 mm x Length 7.5 cm
TSKgel Boronate-5PW Guardgel kit available (P/N: 13125)
 
Coupling of m-aminophenyl boronate to the TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris. 

Applications include: nucleic acids, catecholamines, nucleosides and nucleotides.

 

 Structure:
 



 
Pore size (silica): 1,000 Å 
 
Exclusion limit
(base resin, estimate):		 < 10,000,000 Da
globular proteins
 
Absorption capacity: 40 µmol/mL resin
(sorbitol)
 
Particle size: 10 µm
 
pH stability:
 		 2.0 - 9.0
 
Functional group:
 		 m-aminophenyl 
boronate
 
 

Isocratic separation of nucleosides



 

  • Model: 13066
  • Manufactured by: Tosoh BioScience

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