TSKgel Boronate-5PW Column, 10μm, ID 7.5 mm x Length 7.5 cm
TSKgel Boronate-5PW Guardgel kit available (P/N: 13125)
Coupling of m-aminophenyl boronate to the TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris.
Applications include: nucleic acids, catecholamines, nucleosides and nucleotides.
|Pore size (silica):||1,000 Å |
(base resin, estimate):
|< 10,000,000 Da|
|Absorption capacity:||40 µmol/mL resin|
|Particle size:||10 µm|
|pH stability:||2.0 - 9.0|
|Functional group:||m-aminophenyl |
Isocratic separation of nucleosides