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Tosoh TSKgel Boronate-5PW Glass Guardgel kit, 20 μm - 14451

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Affinity Chromatography

Guardgel kit, 20μm for TSKgel Boronate-5PW Glass Column (P/N 14449) 

Coupling of m-aminophenyl boronate to the TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris. 

Applications include: nucleic acids, catecholamines, nucleosides and nucleotides.

Structure:





Pore size (silica):1,000 Å 

Exclusion limit
(base resin, estimate):		< 10,000,000 Da
globular proteins

Absorption capacity:40 µmol/mL resin
(sorbitol)

Particle size:10 µm

pH stability:

2.0 - 9.0

Functional group:

m-aminophenyl 
boronate



Isocratic separation of nucleosides





  • Model: 14451
  • Manufactured by: Tosoh BioScience

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