Tosoh TSKgel Boronate-5PW Guardgel kit - 13125

Guardgel kit, 20μm, Stainless Steel for TSKgel Boronate-5PW Column (P/N 13066)

Coupling of m-aminophenyl boronate to the TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg²⁺ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris. 

Applications include: nucleic acids, catecholamines, nucleosides and nucleotides.

Structure: Pore size (silica): 1,000 Å  Exclusion limit (base resin, estimate): < 10,000,000 Da globular proteins Absorption capacity: 40 µmol/mL resin (sorbitol) Particle size: 10 µm pH stability: 2.0 - 9.0 Functional group: m-aminophenyl  boronate

Isocratic separation of nucleosides