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Tosoh TSKgel Tresyl-5PW Column, 10 μm, ID 7.5 mm x L 7.5 cm - 14456

Affinity Chromatography

TSKgel Tresyl-5PW Column, 10 μm, ID 7.5 mm x Length 7.5 cm

Unlike other TSKgel affinity columns, TSKgel Tresyl-5PW, which is derivatized with the 2,2,2-trifluroethanesulfonyl ligand, requires activation with a user-selected ligand containing amino, thiol, phenol, or imidazole groups.  The resulting structure is literally a "custom" affinity ligand with excellent pH stability and minimal ligand loss due to leaching. TSKgel Tresyl-5PW readily reacts with amino or thiol groups to form stable covalent alkylamines or thioethers.

 Applications include: glycoproteins, antigens, etc.

 Structure:





Pore size (mean):1,000 Å 

Particle size (mean):10 µm 

pH stability:2.0 - 12.0 

Ligand conc.:ca. 20 µmol/mL resin

Adsorption capacity:

> 60 mg/g dry resin 
(coupling capacity w/
soybean trypsin inhibitor)

Exclusion limit
(base resin, estimate):
< 10,000,000 Da
globular proteins

Active group: 

tresyl


Purification of peroxidase on concanavalin A 
coupled to TSKgel Tresyl-5PW





  • Model: 14456
  • Manufactured by: Tosoh BioScience