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Tosoh TSKgel Amide-80 Normal Phase / HILIC Column, 5 µm, ID 2 mm x L 25 cm - 19697

Normal Phase / Hydrophilic Interaction

TSKgel Amide-80 HPLC Column, 5 µm,  ID 2 mm x Length 25 cm

TSKgel Amide-80 columns are packed with 3, 5, or 10µm spherical silica particles that are covalently bonded with carbamoyl groups. The amide stationary phase provides a unique selectivity under regular normal phase conditions or in the hydrophilic interaction mode of chromatography.  Target applications include the analysis of saccharides, glycans, oligosaccarides, peptides and polar compounds from natural products. Unique advantages of TSKgel Amide-80 phase for saccharide analysis include novel hydrogen bonding retention mechanism between hydroxyl groups of the sample and the carbamoyl group in the stationary phase. The stationary phase does not react with reducing sugars and can be used at elevated temperatures (4 to 80 °C) to prevent peak splitting of carbohydrates that can occur at lower temperatures. In addition, the new, high efficiency, 3µm columns offer shorter analysis time and enhanced sensitivity for LC-MS applications. 

The pore size of the bonded phase is indicated by the number in the product description, in this case TSKgel Amide-80 has 80Å nominal pore size.  The nominal pore size of the starting base silica is 100Å.


Pore size (silica):80 Å 

Particle size:3, 5 and 10 μm 

pH stability:2.0 - 7.5

Functional group:Carbamoyl

Max. temperature:80 °C (50°C for 3 µm)

Separation of polyalcohols on TSKgel Amide-80

Separation of pyridylaminated N-glycans from mouse brain on TSKgel Amide-80, 3 µm

  • Model: 19697
  • Manufactured by: Tosoh BioScience