Tosoh TSKgel NH2-100 Guard Cartridge for 4.6 mm ID Columns, 3/Pkg, 3 μm, ID 3.2 mm x L 1.5 cm - 21972

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Normal Phase / Hydrophilic Interaction

TSKgel NH2-100 Guard Cartridge for 4.6 mm ID Columns, 3 μm, ID 3.2 mm x Length 1.5 cm - Pack of 3

Requires Guard Cartridge Holder P/N: 19018

The new TSKgel NH2-100 amino columns expand the selectivity range of TSKgel HILIC solutions. It is well suited for all separations requiring amino functional groups. In contrast to conventional silica-based amino phases it offers expanded stability under HILIC conditions, the prerequisite for high reproducibility and reliable results. TSKgel NH2-100 is suited for the analysis of all types of hydrophilic compounds like carbohydrates, vitamines or polar metabolites.

The TSKgel NH2-100 HILIC phase is based on a 3 µm silica particle with 100 Å pores, which is treated with a special endcapping procedure. Aminoalkyl groups are introduced step wisely after endcapping. The amino groups act as HILIC functional groups without any peak splits. Due to a high ligand density and large surface area TSKgel NH2-100V 3 µm columns show high retention for very polar compounds.

Structure:   Pore size (silica): 100 Å  Particle size: 3 μm  pH stability: 2.0 - 7.5 Functional group: Aminoalkyl Max. temperature: 80 °C (50°C for 3 µm)

Comparison of selectivity. Separation of water soluble vitamins on a TSKgel Amide-80 and TSKgel NH2-100 column  Columns:        TSKgel NH2-100V 3 μm,                         4.6 mm i.d. × 15 cm L                        TSKgel Amide-80 3 μm,                         4.6 mm i.d. × 15 cm L Peaks:            1 = nicotinamide; 2 = vitamin B2;                        3 = pyridoxine; 4 = nicotinic acid;                         5 = vitamin C; 6 = vitamin B1;                         7 = vitamin B12 Mobile phase: 25 mM phosphate buffer                         (pH 2.5) / ACN = 30/70 Flow rate:      1 mL/min Temperature:  40 °C Detection:       UV @ 254 nm

• Model: 21972
• Manufactured by: Tosoh BioScience

Normal Phase / Hydrophilic Interaction

TSKgel NH2-100 Guard Cartridge for 4.6 mm ID Columns, 3 μm, ID 3.2 mm x Length 1.5 cm - Pack of 3

Requires Guard Cartridge Holder P/N: 19018

The new TSKgel NH2-100 amino columns expand the selectivity range of TSKgel HILIC solutions. It is well suited for all separations requiring amino functional groups. In contrast to conventional silica-based amino phases it offers expanded stability under HILIC conditions, the prerequisite for high reproducibility and reliable results. TSKgel NH2-100 is suited for the analysis of all types of hydrophilic compounds like carbohydrates, vitamines or polar metabolites.

The TSKgel NH2-100 HILIC phase is based on a 3 µm silica particle with 100 Å pores, which is treated with a special endcapping procedure. Aminoalkyl groups are introduced step wisely after endcapping. The amino groups act as HILIC functional groups without any peak splits. Due to a high ligand density and large surface area TSKgel NH2-100V 3 µm columns show high retention for very polar compounds.

Structure:   Pore size (silica): 100 Å  Particle size: 3 μm  pH stability: 2.0 - 7.5 Functional group: Aminoalkyl Max. temperature: 80 °C (50°C for 3 µm)

Comparison of selectivity. Separation of water soluble vitamins on a TSKgel Amide-80 and TSKgel NH2-100 column  Columns:        TSKgel NH2-100V 3 μm,                         4.6 mm i.d. × 15 cm L                        TSKgel Amide-80 3 μm,                         4.6 mm i.d. × 15 cm L Peaks:            1 = nicotinamide; 2 = vitamin B2;                        3 = pyridoxine; 4 = nicotinic acid;                         5 = vitamin C; 6 = vitamin B1;                         7 = vitamin B12 Mobile phase: 25 mM phosphate buffer                         (pH 2.5) / ACN = 30/70 Flow rate:      1 mL/min Temperature:  40 °C Detection:       UV @ 254 nm

• Model: 21972
• Manufactured by: Tosoh BioScience

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