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Tosoh TSKgel ODS-140HTP UPLC/HPLC Column, 2.3 μm, ID 2.1 mm x L 5 cm - 21927

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Reversed Phase Columns: Silica-based

TSKgel ODS-140HTP UPLC / HPLC Column, 2.3 μm, Inner Diameter  2.1 mm x Length 5 cm

TSKgel ODS-140HTP columns provide high resolution and short analyses time at moderate pressures, enabling high throughput separations. The polylayer bonding chemistry of the 2.3 µm particle size of these columns results in highly efficient and durable columns. The lower pressure drop reduces the burden on the hardware, allowing the TSKgel ODS-140HTP columns to be used with either UPLC® (up to 9000 psi) or conventional HPLC systems. 

For use in high throughput applications, including drug discovery, pharmacokinetics and peptide digest separations, TSKgel ODS-140HTP columns offer excellent peak shape for basic compounds.





Pore size (silica):140 Å 

Endcapped:yes

Particle size:2.3 μm

pH stability:2.0 - 7.5

Functional group:C18 (polymeric 
bonding chemistry)

% carbon8%


Column stability:

TSKgel ODS-140HTP columns are stable at high flow rates under demanding step gradient conditions.

Figure A shows that consistent theoretical plate values were obtained on the TSKgel ODS-140HTP column during 1110 gradient cycles consisting of five minute step gradients from 10% to 50% and from 50% to 100% methanol at 0.6 mL/min. During each cycle, pressure fluctuated between 30 and 60 MPa.

Figure A:




Figure B shows injections of test solutes after the first step gradient cycle and after 1110 cycles. The results clearly demonstrate the durability of the TSKgel ODS-140HTP columns when operated at high flow rate and high pressure.

Figure B:


  • Model: 21927
  • Manufactured by: Tosoh BioScience

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