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Tosoh TSKgel G3000SW Silica-based GFC Column, 13 μm, ID 21.5 mm x L 30 cm - 06728

Gel Filtration Chromatography

TSKgel G3000SW Stainless Steel Silica-based GFC Column, 10 μm, ID 7.5 mm x Length 60 cm

TSKgel SW columns, introduced in 1977, were the first of a long line of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology. 

Particle Characteristics

TSKgel SW-type columns are based on highly porous silica particles, the surface of which has been shielded from interacting with proteins by derivatization with ligands containing diol functional groups. TSKgel SW-type columns stand out from other silica- or polymer-based high performance size exclusion columns by virtue of their large pore volumes.

Calibration Curves

The figure below shows the calibration curves for globular proteins, polyethylene oxides and dextrans for each of the three TSKgel SW-type columns. The following table summarizes the molecular weight ranges for TSKgel G2000SW, TSKgel G3000SW and TSKgel G4000SW columns for globular proteins, polyethylene oxides and dextrans that can, at least partially, access the particle pores. For comparative purposes, similar data is provided as well for TSKgel SWxl and TSKgel SuperSW-type columns.

As the first generation of silica-based GFC columns, TSKgel G3000SW columns provide excellent separation of proteins and other biomolecules with molecular weights up to 500,000 Da.

The TSKgel G3000SW columns start at 10 μm for analytical columns and also scale to 13 μin columns up to 21.5 mm ID for preparative purifications.

Polyethylene oxide, dextran and protein calibration curves for TSKgel SW columns

 Product Attributes:
Base material:Silica

Particle size (mean):10 and 13 μm

Pore size:250 Å

Functional group:Diol

pH stability:2.5 - 7.5

Calibration range:2,000-5x10^5 Da (globular proteins)

Comparison of the pore sizes of TSKgel SW packings for the separation of a protein mixture

  • Model: 06728
  • Manufactured by: Tosoh BioScience