Gel Filtration Chromatography
TSKgel G4000SW Glass Silica-based SEC Column, 13 μm, ID 8 mm x Length 30 cm
Glass Guard Column Available P/N: 08805
TSKgel SW columns, introduced in 1977, were the first of a long line of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology.
TSKgel SW-type columns are based on highly porous silica particles, the surface of which has been shielded from interacting with proteins by derivatization with ligands containing diol functional groups. TSKgel SW-type columns stand out from other silica- or polymer-based high performance size exclusion columns by virtue of their large pore volumes.
The figure below shows the calibration curves for globular proteins, polyethylene oxides and dextrans for each of the three TSKgel SW-type columns. The following table summarizes the molecular weight ranges for TSKgel G2000SW, TSKgel G3000SW and TSKgel G4000SW columns for globular proteins, polyethylene oxides and dextrans that can, at least partially, access the particle pores. For comparative purposes, similar data is provided as well for TSKgel SWxl and TSKgel SuperSW-type columns.
As the first generation of silicia based SEC columns, the TSKgelG4000SW columns provide excellent separation of proteins and other biomolecules.
TSKgel G4000SW columns are available in 13 μm particle size in Glass and Stainless Steel analytical columns. Particle sizes of 13 and 17 μm are used to pack 30 and 60 cm L x 21.5 mm ID columns, respectively, for semi-preparative purifications.
|Polyethylene oxide, dextran and protein calibration curves for TSKgel SW columns|
- Model: 08801
- Manufactured by: Tosoh BioScience