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Tosoh TSKgel TSKgel SuperSW3000 HPLC Column, 4 μm, ID 2 mm x L 30 cm - 21485

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Gel Filtration Chromatography

TSKgel TSKgel SuperSW3000 HPLC Column, 4 μm, ID 2 mm x Length 30 cm

TSKgel SuperSW columns, introduced in 1997, represent the third generation of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology. 

Particle Characteristics
TSKgel SuperSW-type columns contain smaller particles than SWXL-type columns; 4 micron versus 5 micron. In addition, the column internal diameter has been reduced from 7.8 mm ID to 4.6 mm ID to provide higher sensitivity in sample-limited cases and to cut down on solvent use.

HPLC System Requirements
It is important to employ an HPLC system that is optimized with regards to extra-column bandbroadening to take full advantage of the high column efficiency that can be obtained on the TSKgel SuperSW columns. See Separation Report 95 for recommendations.

Featuring ultra-efficient 4 μm particles, TSKgel SuperSW3000 provides higher efficiency than 5 μmTSKgel G3000SWxl columns. The 250 Å pore size results in a fractionation range up to 500,000 Da for globular proteins. The narrow 4.6 mm column inner diameter reduces buffer consumption and is ideal for sample-limited applications. 

To fully benefit from the high efficiency of 4 μm columns, and because the column diameter of TSKgel SuperSW columns is 4.6 mm or less, it is important to minimize the amount of dead volume in the HPLC system. Consult the Separation Report for details (provided below).


 Product Attributes:
Base material:Silica

Particle size (mean):4 μm

Pore size:250 Å

Functional group:Diol

pH stability:2.5 - 7.5

Calibration range:10,000-500,000 Da (globular proteins)



Overlays of monoclonal antibody separation with 0.2 M NaCl, 0.2 M NaClO4 and 0.4 M NaClO4

(Enlargement of fusion protein 1's baseline region shows improved peak shape of the dimer and trimer aggregates with 0.4 M NaClO4)




Published with permission of Scott Lauder, Lexigen Pharmaceuticals


  • Model: 21485
  • Manufactured by: Tosoh BioScience

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