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Tosoh TSKgel G-DNA-PW Aqueous SEC Column, 10 μm, ID 7.8 mm x L 30 cm - 08032

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Gel Filtration Chromatography

TSKgel G-DNA-PW Aqueous SEC Column, 10 μm, ID 7.8 mm x Length 30 cm
Guard Column Available - P/N: 08033
Top-Off for PWxl, 1 g Available - P/N: 08035

Tosoh's polymer-based gel filtration columns are either prepared from polymethacrylate resin (TSKgel PW, PWXL and PWXL-CP) or from hydroxylated polyvinyl (TSKgel Alpha and SuperAW) resin. Each resin type can be synthesized in a wide variety of different pore sizes. The four major product groups also differ from one another in average particle size. A more important distinction is, however, the range of solvents that can be used with each product line.

Hydroxylated polymethacrylate beads

  • From water to 20% (under some conditions 50%) organic solvents

    • TSKgel PWXL and 
      TSKgel PWXL-CP, 7-13 
      μm
    • TSKgel PW, 12-17 μm

Hydroxylated polyvinyl beads

  • Water and most polar organic solvents

    • TSKgel SuperAW, 4-9 μm
    • TSKgel Alpha, 7-13 μm

Each product group contains columns with distinctly different pore sizes, and one mixed-bed column.

TSKgel PWxl columns are composed of spherical, hydrophilic polymethacrylate beads. The main application area for TSKgel PWxl columns is the analysis of water-soluble polymers, such as celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides. See the table on the TSKgel PW page for typical samples and suitable eluents. Figure 1 shows the calibration curves for all TSKgel PWxl-type columns. Figure 2 shows the benefits of TSKgel PWxl columns over PW columns, namely the improved resolution due to the smaller particle sizes used in PWxl columns.

TSKgel G-DNA-PW is a specialty column designed for the separation of large polynucleotides such as DNA and RNA fragments of 500-5000 base pairs. TSKgel G-DNA-PW is a smaller particle size version of TSKgel G6000PWxl.

Product Attributes:
Base materialhydroxylated polymethacrylate

Particle size (mean):10 μm

Pore size:> 1,000 Å

Working range:

500 - 5000 bp DNA
Max. % organic: 20%

Shipping solvent:water



Calibration curve for double-stranded DNA



Separation of large DNA fragments on a 
TSKgel G-DNA-PW column


  • Model: 08032
  • Manufactured by: Tosoh BioScience