Gel Filtration Chromatography
TSKgel GMPW Aqueous SEC Column, 17 μm, ID 7.5 mm x Length 60 cm
Guard Column Available - P/N: 06762
Tosoh's polymer-based gel filtration columns are either prepared from polymethacrylate resin (TSKgel PW, PWXL and PWXL-CP) or from hydroxylated polyvinyl (TSKgel Alpha and SuperAW) resin. Each resin type can be synthesized in a wide variety of different pore sizes. The four major product groups also differ from one another in average particle size. A more important distinction is, however, the range of solvents that can be used with each product line.
Hydroxylated polymethacrylate beads
- From water to 20% (under some conditions 50%) organic solvents
- TSKgel PWXL and
TSKgel PWXL-CP, 7-13 μm
- TSKgel PW, 12-17 μm
Hydroxylated polyvinyl beads
- Water and most polar organic solvents
- TSKgel SuperAW, 4-9 μm
- TSKgel Alpha, 7-13 μm
Each product group contains columns with distinctly different pore sizes, and one mixed-bed column.
TSKgel PW columns are composed of spherical, hydrophilic polymethacrylate beads. Particle sizes range from 12 µm for the smaller pore size columns to 17 µm for the larger pore size columns. TSKgel GMPW is a mixed bed scouting column for water-soluble linear polymers. The pore volume can be accessed by polymers ranging in molecular weight from 500 to 8,000,000 Da. By quickly categorizing the MW profile of an unknown sample, the column enables a fast selection of the best TSKgel PW column for routine analysis. Mixed bed columns are also used when a sample has a very broad MW distribution.
|Base material:||hydroxylated polymethacrylate|
|Particle size (mean):||17 μm|
|Pore size:||< 100 Å - 1000 Å|
|Calibration range: ||500 - 8,000,000 Da |
(polyethylene glycols &
|Max. % organic: ||20%|
|Effect of ionic strength on th elution of anionic polymers|