TSKgel PW Columns (Type of Polymer) for Analysis of Water-Soluble Polymers
TSKgel G2500PW - GMPW Guard Column for 7.5 mm ID columns, 12μm, Stainless Steel, ID 7.5 mm x L 75 mm
Part Number: 06762
TSKgel PW columns are composed of spherical, hydrophilic polymethacrylate beads. Particle sizes range from 12 µm for the smaller pore size columns to 17 µm for the larger pore size columns.
The main application areas for TSKgel PW columns are the analysis of water-soluble polymers, such as celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides.
Recommended eluents for GFC of water-soluble polymers on TSKgel PW columns:
Type of Polymer | Typical Sample | Suitable Eluent |
Nonionic hydrophilic | polyyethylene glycol | distilled water |
Anionic Hydrophilic | sodium chondroitin sulfate, sodium alginate, carboymethyl cellulose, sodium polyacrylate, sodium hyaluronate | Buffer or salt solution (e.g. 0.1 mol/L NaNO3) |
Anionic Hydrophobic | sulfonated lignin sodium salt, sodium polystyrenesulfonate | Buffer or salt solution with organic solvent (e.g. 20% CH3CN in 0.1 mol/L NaNO3) |
Cationic Hydrophilic | glycol chitosan, DEAE-dextran, poly(ethyleneimine), poly(trimethylaminoethyl methacrylate) iodide salt | 0.5 mol/L acetic acid with 0.3 mol/L Na2SO4 or 0.8 mol/L NaNO3 |
Cationic Hydrophobic | poly(4-vinylbenzyltrimethylammonium chloride), poly(N-methyl-2-vinylpyridinium) iodide salt | 0.5 mol/L acetic acid with 0.3 mol/L Na2SO4 |
Amphoteric Hydrophilic | peptides, proteins, poly- and oligosaccharides, DNA, RNA | Buffer or salt solution (e.g. 0.1 mol/L NaNO3) |
Amphoteric hydrophobic | blue dextran, collagen, gelatin, hydrophobic proteins, hydrophobic peptides | Buffer or salt solution with organic solvent (e.g. 20% CH3CN in 0.1 mol/L NaNO3 or 35-45% CH3CN in 0.1% TFA) |
- Model: 06762
- Manufactured by: Tosoh BioScience