Waters MaxPeak Premier Protein SEC Guard, 250A, 2.5 um, 4.6 x 30 mm, 1/pk - 186009969

Waters™ MaxPeak Premier Protein SEC Guard, 250Å, 2.5 µm, 4.6 x 30 mm, 1/pk

Product Number: 186009969

Specifications:

MaxPeak Premier Protein SEC 250Å 2.5 µm Guard Columns were developed to  help scientists extend the column life of either an ACQUITY Premier Protein SEC 250Å, 1.7 µm Column or an XBridge Premier Protein SEC 250Å, 2.5 µm Column without degrading desired separated component resolution. The useful lifetime of the guard column can be influenced by many factors including mobile phase cleanliness/microbial contaminatio , presence of sample precipitates/aggregates, working at extremes of pressure, pH, and/or temperature. Guard columns may need replacement if significant increase in column pressure is observed or if one sees wide tailing or split peaks. 

MaxPeak Premier Protein SEC Columns

More confident biological compound separations start here.

Get superior size-based separations of biological compounds with MaxPeak Premier Protein SEC Columns.

Size-exclusion chromatography (SEC) is a powerful technique for aggregate and fragment analysis in the research, development, and manufacturing of biotherapeutic proteins, such as insulin and monoclonal antibodies (mAb). 

Experience unmatched batch-to-batch reproducibility with the wide range of Waters SEC columns and standards for peptides and proteins characterization.

• Get 30% improved resolution with Waters MaxPeak Premier Protein SEC Columns
• Eliminate the need for extensive method development with a one-size-fits-all method in your lab
• Get unmatched batch-to-batch reproducibility with the wide range of Waters SEC columns and standards for peptide and protein characterization
• Limit unwanted secondary interactions that lead to broader peaks, inaccurate quantitation of aggregates, and increased tailing
• Recommended Use: Platform method for protein size variant analysis from 10-650 KDa

30% improved resolution

• MaxPeak Premier Columns deliver highly efficient separations that enable you to resolve species that vary by less than 2X in size on any LC
• Achieve narrow peak widths with reduced tailing providing accurate quantitation of aggregates and fragments of <0.5% total peak areas and 30% better resolution
• Gain efficiency for proteins ranging from 10 KDa to 650 KDa, leveraging MaxPeak Premier Technology coupled with BEH-PEO Particle Technology

More confident platform methods

• Eliminate the need for extensive method development by using a one-size- fits-all method in your lab
• Ensure accurate results, such as high molecular weight (HMW) percentage, which should not depend on mobile phase choice
• Resolve protein size variants for over 80% of proteins without the need for additives with a Phosphate Buffered Saline (PBS) eluent

Exceedingly inert for biologics

SEC should separate proteins based exclusively on size, however unwanted secondary interactions can lead to broader peaks, inaccurate quantitation of aggregates, and increased tailing. Waters has industry-leading innovations to limit secondary interactions on both column and particles:

• MaxPeak High Performance Surface columns mitigates ionic interactions between hardware and proteins
• Ethylene Bridged Hybrid (BEH) SEC particles with high coverage hydroxy-terminated polyethylene oxide (PEO) bonding minimizes hydrophobic particle interactions
• MaxPeak High Performance Surfaces enable out-of-the-box performance reducing the need for lengthy column conditioning typically observed in other columns

MaxPeak Premier Columns

See all of your sample with MaxPeak Premier Columns, the most universal column platform for chromatographers who need to reduce variability risks and save time, all while increasing recovery and sensitivity.

Non-specific adsorption (NSA) of metal sensitive compounds is an unpredictable challenge, leading to long column passivation times, chromatography with large relative standard deviations (RSDs), and broad peaks that can be a challenge to detect.

See and do more with MaxPeak Premier Columns, which utilize the latest progressive integrated technologies like MaxPeak High Performance Surfaces (HPS) to effectively reduce non-specific adsorption (NSA) losses due to metal interactions.

With MaxPeak Premier Columns, your lab can reduce the time wasted on column passivation and method optimization, while increasing reproducibility, sensitivity, and overall quality of results. 

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