Waters ACQUITY Premier Glycoprotein BEH Amide, 300 Å, 1.7 µm, 2.1 x 20 mm Column, 1/pk
Part Number: 186011017
The ACQUITY Premier Glycoprotein BEH Amide 300 Å, 1.7 µm, 2.1 x 20 mm Column is designed for the analysis of intact glycoproteins, glycoprotein fragments, and glycopeptides. Each batch of Glycoprotein BEH Amide 300Å, 1.7 µm, material is specifically quality control tested with the Waters Glycoprotein Performance Test Standard (P/N: 186008010) that includes a mixture of Ribonuclease A as well as Ribonulease B glycoforms to help ensure batch to batch consistency. The column hardware utilizes MaxPeak High Performance Surface (HPS) that reduces unwanted analyte surface interactions and greatly improves peak shape, recovery, and reproducibility. Suggested use: Glycoprotein analysis
ACQUITY Premier Columns
Stop looking and start seeing – no compromise required
Reduce the risks associated with unpredictable chromatography with ACQUITY Premier Columns with MaxPeak High Performance Surfaces (HPS) Technology, which allow you to see and do more by reducing analyte/surface interactions.
With Waters ACQUITY Premier Columns, chromatographers can harness the separation efficiency improvements and increased flow rates of ultra-performance liquid chromatography (UPLC) technology, improving analyte resolution, sensitivity, business productivity, and profitability. As the next evolution in the ACQUITY UPLC Column family, ACQUITY Premier Columns are built on the industry-leading sub-2-µm particle technologies from Waters you trust.
- Reduces column conditioning and passivation for higher productivity
- Increases reproducibility from analysis to analysis, for more confidence in analytical results
- Reduces non-specific absorption for increased sensitivity, improved peak shape, and easier analyte integration
- Provides superior separation speed, sensitivity, and resolution with best-in-class column efficiency
- 3 UPLC particles of various substrate technologies, including Ethylene Bridged Hybrid (BEH), High Strength Silica (HSS), and Charged Surface Hybrid (CSH)
- Reversed-phase and HILIC stationary phases, including C18, Embedded-Polar, and Amide
- Application-specific column chemistries for peptides, proteins, oligonucleotides, and glycan analysis
- Prolonged column lifetimes with VanGuard FIT Columns, and easy-to-use, all-in-one guard/column design
- Recommended Use: For increased sensitivity, improved peak shape, and less column equilibration time in methods development.
ACQUITY Premier BEH Amide Columns
- Designed to retain highly polar analytes that are too polar to retain by reversed-phase
- Exceptional chemical stability at high pH and high temperature
- Ideal for sugar/carbohydrate analysis
- Exceptional retention of polar analytes spanning a wide range of polarity
BEH (Ethylene Bridged Hybrid) Technology
Ethylene Bridged Hybrid (BEH) particle technology ensures maximum column performance and longer column lifetimes under all chromatographic conditions. The hybrid particle technology offers many advantages over conventional silica-based particles, including the ability to control silanol activity for better reproducibility, peak shape, and efficiency. The BEH particle is created from two high purity monomers: tetraethoxysilane (TEOS) and bis(triethoxysilyl) ethane (BTEE), which results in highly stable, pH resistant, and mechanically strong particles that set the performance standard for method development. BEH particle technology allows for seamless transfer from analytical to preparative separations.
The advantages of BEH columns include:
- Rugged hybrid organic/inorganic base particle
- Improved peak shape for bases-low silanol activity
- Wider operating pH range for method development flexibly
- Mechanical strength for maximum column efficiency at UHPLC operating pressures
- Available Particle Sizes: 1.7, 2.5, 3.5, 5, and 10 µm
- Column Chemistries: C18, C18 AX, Shield RP18, C8, Phenyl, Amide, Z-HILIC, HILIC
Related Products:
- 186009547 - ACQUITY Premier Glycoprotein BEH Amide, 300Å, 1.7 µm, 2.1 x 50 mm Column, 1/pk
- 186009548 - ACQUITY Premier Glycoprotein BEH Amide, 300Å, 1.7 µm, 2.1 x 100 mm Column, 1/pk
- 186009549 - ACQUITY Premier Glycoprotein BEH Amide, 300Å, 1.7 µm, 2.1 x 150 mm Column, 1/pk
Specifications:
Chemistry | Amide |
Separation Mode | Hydrophilic Interaction (HILIC) |
Particle Substrate | Hybrid |
pH Range Min | 2 pH |
pH Range Max | 10 pH |
Temperature Limits | 90 C |
Maximum Pressure | 15000 psi (1034 Bar) |
Endcapped | No |
Silanol Activity | Low |
Particle Shape | Spherical |
Particle Size | 1.7 µm |
Endfitting Type | Waters |
Pore Size | 300 Å |
QC Tested | Glycoprotein |
Format | Premier Column |
Surface Area | 185 |
System | UHPLC, UPLC |
Particle Technology | BEH |
Technique | LC/MS |
USP Classification | L68 |
Inner Diameter | 2.1 mm |
Length | 20 mm |
Carbon Load | 12% |
UNSPSC | 41115709 |
Application | Glycan |
Brand | ACQUITY |
Product Type | Columns |
Units per Package | 1 pk |
- Model: 186011017
- Manufactured by: Waters