Waters ACQUITY Premier Oligonucleotide BEH C18 Column, 130 Å, 1.7 µm, 2.1 x 20 mm 1/pk - 186011115

Waters ACQUITY Premier Oligonucleotide BEH C18 Column, 130 Å, 1.7 µm, 2.1 x 20 mm 1/pk

Part Number: 186011115

The ACQUITY Premier Oligonucleotide BEH C18, 300 Å, 1.7 µm, 2.1 x 20 mm is used to separate oligonucleotides via ion-pairing reversed-phase (IP-RP) chromatography on UPLC systems. Each column incorporates Waters' BEH hybrid particle technology for unmatched pH and temperature stability and our MaxPeak Premier high performance surface technology for ultra-low non-specific adsorption. Stationary phase batches are QC tested using the Waters MassPREP Oligonucleotide Standard to ensure lot-to-lot performance. Premier Oligonucleotide BEH Columns provide unmatched sensitivity, exquisite reproducibility, and excellent out-of-the-box performance. Columns with 130Å pore size particles deliver industry leading performance for short-mer oligonucleotide separations, and new 300 Å pore size columns offer enhanced resolution for longer oligonucleotides.

ACQUITY Premier Oligonucleotide Columns

Suited for the analysis of detritylated oligonucleotides using ion-pair, reversed-phase chromatography
Widepore BEH 300Å C18 columns facilitate long-mer oligo separations for efficient poly A tail analysis and CRISPR sgRNA characterization

ACQUITY Premier Columns

Stop looking and start seeing – no compromise required

Reduce the risks associated with unpredictable chromatography with ACQUITY Premier Columns with MaxPeak High Performance Surfaces (HPS) Technology, which allow you to see and do more by reducing analyte/surface interactions.

With Waters ACQUITY Premier Columns, chromatographers can harness the separation efficiency improvements and increased flow rates of ultra-performance liquid chromatography (UPLC) technology, improving analyte resolution, sensitivity, business productivity, and profitability. As the next evolution in the ACQUITY UPLC Column family, ACQUITY Premier Columns are built on the industry-leading sub-2-µm particle technologies from Waters you trust.

• Reduces column conditioning and passivation for higher productivity
• Increases reproducibility from analysis to analysis, for more confidence in analytical results
• Reduces non-specific absorption for increased sensitivity, improved peak shape, and easier analyte integration
• Provides superior separation speed, sensitivity, and resolution with best-in-class column efficiency
• 3 UPLC particles of various substrate technologies, including Ethylene Bridged Hybrid (BEH), High Strength Silica (HSS), and Charged Surface Hybrid (CSH)
• Reversed-phase and HILIC stationary phases, including C18, Embedded-Polar, and Amide
• Application-specific column chemistries for peptides, proteins, oligonucleotides, and glycan analysis
• Prolonged column lifetimes with VanGuard FIT Columns, and easy-to-use, all-in-one guard/column design
• Recommended Use: For increased sensitivity, improved peak shape, and less column equilibration time in methods development.

ACQUITY Premier BEH C18 Columns

• Universal C18 Column
• Suitable for a diverse range of analytes
• High efficiency
• Industry-leading mobile-phase pH 1–12 and temperature compatibility

BEH (Ethylene Bridged Hybrid) Technology

Ethylene Bridged Hybrid (BEH) particle technology ensures maximum column performance and longer column lifetimes under all chromatographic conditions. The hybrid particle technology offers many advantages over conventional silica-based particles, including the ability to control silanol activity for better reproducibility, peak shape, and efficiency. The BEH particle is created from two high purity monomers: tetraethoxysilane (TEOS) and bis(triethoxysilyl) ethane (BTEE), which results in highly stable, pH resistant, and mechanically strong particles that set the performance standard for method development. BEH particle technology allows for seamless transfer from analytical to preparative separations.

The advantages of BEH columns include:

• Rugged hybrid organic/inorganic base particle
• Improved peak shape for bases-low silanol activity
• Wider operating pH range for method development flexibly
• Mechanical strength for maximum column efficiency at UHPLC operating pressures
• Available Particle Sizes: 1.7, 2.5, 3.5, 5, and 10 µm
• Column Chemistries: C18, C18 AX, Shield RP18, C8, Phenyl, Amide, Z-HILIC, HILIC

Related Products:

• 186004135 - MassPREP Oligonucleotide Standard
• 186009449 - ssDNA 10 to 60 Ladder
• 186009448 - ssDNA 20 to 100 Ladder

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