Waters ACQUITY Premier Protein SEC Column Method Validation Kit, 250 Å, 1.7 µm, 4.6 x 150 mm, 3/pk - 186011142

Waters ACQUITY Premier Protein SEC Column, 250Å, 1.7 µm, 4.6 x 150 mm Method Validation Kit, 3/pk

Part Number: 186011142

ACQUITY Premier Protein SEC Column, 250Å, 1.7 µm, 4.6 x 150 mm Method Validation Kit Columns help scientists obtain reliable protein size variant separations (10,000 to approx 650,000 Daltons) via the synergistic coupling of Waters MaxPeak Premier High Performance Surfaces and novel BEH-PEO Particle technologies. These advancements in SEC column hardware and SEC particle technology work to minimize non-desired secondary ionic or hydrophobic interactions between proteins and the column to minimize costly method development work by allowing chromatographers to use a “generic” or “platform-type” method for many samples. Each batch is specifically QC tested using Waters mAb Size Variant Standard (p/n: 186009429) to help ensure consistent performance for challenging size-based protein separations.

Note: This Method Validation Kit (MVK) contains (3) (P/N 186009959) columns from (3) different batches.

ACQUITY Premier Protein SEC 250 Å Guards and Columns

• Sub-2-μm particle columns for UPLC separations
• 250 Å pore size
• High throughput & resolution SEC separations of biomolecules ranging from 10,000 to 650,000 Daltons on low dispersion UPLC Systems
• Available SEC Guard Columns to extend analytical SEC Column life
• Significantly reduce non-desired ionic and hydrophobic secondary interaction for desired SEC platform methods with MaxPeak Premier Technology and BEH-PEO SEC particle technology

ACQUITY Premier Columns

Stop looking and start seeing – no compromise required

Reduce the risks associated with unpredictable chromatography with ACQUITY Premier Columns with MaxPeak High Performance Surfaces (HPS) Technology, which allow you to see and do more by reducing analyte/surface interactions.

With Waters ACQUITY Premier Columns, chromatographers can harness the separation efficiency improvements and increased flow rates of ultra-performance liquid chromatography (UPLC) technology, improving analyte resolution, sensitivity, business productivity, and profitability. As the next evolution in the ACQUITY UPLC Column family, ACQUITY Premier Columns are built on the industry-leading sub-2-µm particle technologies from Waters you trust.

• Reduces column conditioning and passivation for higher productivity
• Increases reproducibility from analysis to analysis, for more confidence in analytical results
• Reduces non-specific absorption for increased sensitivity, improved peak shape, and easier analyte integration
• Provides superior separation speed, sensitivity, and resolution with best-in-class column efficiency
• 3 UPLC particles of various substrate technologies, including Ethylene Bridged Hybrid (BEH), High Strength Silica (HSS), and Charged Surface Hybrid (CSH)
• Reversed-phase and HILIC stationary phases, including C18, Embedded-Polar, and Amide
• Application-specific column chemistries for peptides, proteins, oligonucleotides, and glycan analysis
• Prolonged column lifetimes with VanGuard FIT Columns, and easy-to-use, all-in-one guard/column design
• Recommended Use: For increased sensitivity, improved peak shape, and less column equilibration time in methods development.

SEC Columns

Size-based separations of peptides, proteins, nucleic acids, LNPs, and viral vectors

Get unmatched batch-to-batch reproducibility with the wide range of Waters Size-Exclusion Chromatography (SEC) Columns and standards for protein and peptide characterization.

SEC is a powerful technique for aggregate and fragment analysis in the research, development, and manufacturing of biotherapeutic proteins, such as insulin and monoclonal antibodies (mAb). With SEC, also known as gel filtration, you can separate proteins based on their size (hydrodynamic radii versus absolute molecular weight) in solution, with larger sized species eluting before smaller proteins.

The pore size and volume of the SEC particles used in the packed column drives this isocratic based technique, so the column you choose matters. As the molecular weight of the proteins of interest increases, SEC columns with comparatively larger pore size particles are selected.

Related Products:

• 186009969 - MaxPeak Premier Protein SEC Guard, 250 Å, 2.5 µm, 4.6 x 30 mm, 1/pk
• 186009429 - mAb Size Variant Standard

Specifications: