Waters ACQUITY UPLC Protein BEH C4 VanGuard Pre-column, 300A, 1.7 um, 2.1 mm X 5 mm, 10K - 500K, 3/pk - 186004623

Waters™ ACQUITY UPLC Protein BEH C4 VanGuard Pre-column, 300Å, 1.7 µm, 2.1 mm X 5 mm, 10K - 500K, 3/pk

Product Number: 186004623

Specifications:

Waters Protein Separation Technology (PrST) columns are QC tested and optimized to separate proteins based on size, hydrophobicity and isoelectric point. If you compare to traditional C18 phases, the C4 ligand is less retentive and will minimize protein carryover, increase protein recovery, and improve peak capacity. The VanGuard pre-column connects directly to the inlet of the ACQUITY UPLC column which minimizes extra columns volumes and reduces leaking by eliminating additional connections.

UPLC Column Protection – VanGuard Pre-Columns

Contamination resulting from the analysis of samples present within complex matrices, or that are particulate-laden, may result in reduced column lifetime if not properly addressed. VanGuard™ Pre-Columns are ideally suited for the physical and chemical protection of ACQUITY UPLC Columns. Directly compatible with UPLC pressures up to 18,000 psi [1241 bar], this ultra-low dispersion direct connect guard column is specifically engineered to preserve the lifetime of an ACQUITY UPLC Column without negatively impacting its separation performance.

BEH (Ethylene Bridged Hybrid) Technology

In 1999, Waters launched the XTerra family of HPLC columns featuring first generation hybrid particle technology (HPT). HPT enabled XTerra columns to become one of the most successful column products in the history of Waters. In HPT, the best properties of inorganic (silica) and organic (polymeric) packings are combined to produce a material that has superior mechanical strength, efficiency, high pH stability and peak shape for bases. The first generation methyl hybrid particles of XTerra columns did not possess the mechanical strength or efficiency necessary to fully realize the potential speed, sensitivity and resolution capabilities of UPLC technology. Therefore, a new pressure-tolerant particle needed to be created. A new, second generation hybrid material was developed which utilizes an ethylene bridged hybrid (BEH) structure. Compared to the first generation methyl hybrid particle of XTerra columns, the BEH particle of ACQUITY UPLC BEH columns exhibits improved efficiency, strength and pH range. BEH Technology™ is a key enabler behind the speed, sensitivity and resolution of UPLC separations.

ACQUITY UPLC columns

ACQUITY UPLC columns are the most technologically advanced LC columns ever created and are the only columns designed, tested and certified to withstand the pressures of UPLC Technology. Based upon the highly efficient 1.7 µm Bridged Ethyl Hybrid (BEH) particles and 1.8 µm High Strength Silica (HSS) particles, these columns are the first and only columns that allow separation scientists to achieve maximum speed, sensitivity and resolution without compromise. The particles and chemistries of the ACQUITY UPLC column family were carefully chosen to produce the ideal combination of ultra-performance efficiencies, wide pH range and complementary selectivities. ACQUITY UPLC BEH columns and ACQUITY UPLC HSS columns are the only columns recommended for use with ACQUITY UPLC Systems.

There is more to creating a UPLC particle than synthesizing a small particle. Many HPLC particles do not possess the mechanical stability and structural integrity to withstand UPLC operating pressures (e.g., 15000 psi/1000 bar). Why is pressure tolerance important? In order to realize the efficiency gains of sub-2 μm particles, the ability to routinely operate at higher linear velocities (e.g., higher flow rates) is required. These higher linear velocities combined with small, sub-2 μm particles result in higher operating backpressures. Waters has created two highly efficient, pressure-tolerant UPLC particles: the 1.7 μm Ethylene Bridged Hybrid (BEH) particle and the 1.8 μm High Strength Silica (HSS) particle.

The first ACQUITY UPLC particle created was the 1.7 μm Ethylene Bridged Hybrid (BEH) particle. This second generation hybrid particle is one of the key enablers behind UPLC technology and is available in five column chemistries: C18, C8, Shield RP18, Phenyl and HILIC. Because this is a hybrid particle, a wider usable pH range (up to pH 1-12) makes method development faster and easier. BEH particles are also available in HPLC particle sizes (2.5, 3.5, 5 and 10 μm) in the XBridge family of HPLC columns, thus allowing seamless transfer between HPLC and UPLC separations.

ACQUITY UPLC HSS column chemistries include HSS C18, HSS C18 SB (Selectivity for Bases) and T3. The HSS C18 chemistry is a fully endcapped, ultra-performance, general purpose C18 bonded phase that provides superior peak shape for bases, increased retentivity (vs. ACQUITY UPLC BEH C18 columns), and extremely long lifetimes under acidic conditions. The HSS C18 SB (Selectivity for Bases) chemistry is an unendcapped C18 bonded phase designed and optimized for low pH method development and offers alternate selectivities, especially for basic com- pounds, as compared to most modern, high coverage C18 chemistries. The HSS T3 chemistry is an aqueous mobile phase compatible C18 bonded phase that is designed to retain and separate small, water soluble, polar organic molecules, much like Atlantis T3 HPLC columns.