Welcome, Sign In First time visitor? Create a New Account
Your cart is empty

Waters ACQUITY UPLC Oligonucleotide (OST) BEH C18 Column, 130 Å, 1.7 µm, 2.1 mm X 50 mm, 1K - 30K, 1/pk - 186003949

Price: £881.00


Qty. 
+-

Waters ACQUITY UPLC Oligonucleotide BEH C18 Column, 130Å, 1.7 µm, 2.1 mm X 50 mm, 1K - 30K, 1/pk

Product Number: 186003949

Waters Oligonucleotide Separation Technology (OST) columns are used to separate detritylated synthetic oligonucleotides based on ion-pair, reversed-phase chromatography. Each column is QC tested using Waters MassPrep OST standards to ensure repeatable performance for your most demanding oligonucleotide assays.acqui.

Oligonucleotide Reversed-Phase Columns

Ion pairing reversed-phase (IPRP) chromatography is the method of choice for synthetic oligonucleotide characterization and impurities analysis. The method helps scientists resolve synthesis and process-related impurities better than any other chromatographic technique, including anion exchange (AEX), HILIC, and SEC. The technique is not without its challenges. Multiple factors can directly affect separation performance and reproducibility, including the purity and stability of ion-pairing reagents, non-specific adsorption to chromatographic hardware, and batch-to-batch variations in sorbent. IPRP relies on high pH and temperature conditions for optimal separations, requiring column materials to have high pH and temperature tolerance.

Gain confidence in your results using Waters Oligonucleotide Reversed Phase Columns that offer:

  • Industry-leading pH and temperature stability with BEH C18 hybrid silica particle technology for exceptional resolution and column life.
  • A dramatic reduction of non-specific adsorption (NSA) with MaxPeak Premier High-Performance Surfaces
  • (HPS) Technology for maximum sensitivity, reproducibility, and productivity.
  • Oligonucleotide-based QC testing to ensure lot-to-lot separations performance and reproducibility.

BEH (Ethylene Bridged Hybrid) Technology

Ethylene Bridged Hybrid (BEH) particle technology ensures maximum column performance and longer column lifetimes under all chromatographic conditions. The hybrid particle technology offers many advantages over conventional silica-based particles, including the ability to control silanol activity for better reproducibility, peak shape, and efficiency. The BEH particle is created from two high purity monomers: tetraethoxysilane (TEOS) and bis(triethoxysilyl) ethane (BTEE), which results in highly stable, pH resistant, and mechanically strong particles that set the performance standard for method development. BEH particle technology allows for seamless transfer from analytical to preparative separations.

Specifications

ChemistryC18
Separation ModeReversed Phase
Particle SubstrateHybrid
pH Range Min1 pH
pH Range Max12 pH
EndcappedYes
Silanol ActivityLow
Molecular Weight Range Min1000
Molecular Weight Range Max30000
Particle ShapeSpherical
Particle Size1.7 µm
Endfitting TypeParker-style
Pore Size130 Å
QC TestedOligonucleotide
FormatColumn
SystemUHPLC, UPLC
Particle TechnologyBEH
USP ClassificationL1
Inner Diameter2.1 mm
Length50 mm
Carbon Load18%
UNSPSC41115709
ApplicationOligonucleotide, Gene Therapeutics
BrandACQUITY UPLC
Product TypeColumns
Units per Package1 pk

  • Model: 186003949
  • Manufactured by: Waters