Waters ACQUITY UPLC Oligonucleotide BEH C18 Column, 130Å, 1.7 µm, 2.1 mm X 50 mm, 1K - 30K, 1/pk
Product Number: 186003949
Waters Oligonucleotide Separation Technology (OST) columns are used to separate detritylated synthetic oligonucleotides based on ion-pair, reversed-phase chromatography. Each column is QC tested using Waters MassPrep OST standards to ensure repeatable performance for your most demanding oligonucleotide assays.acqui.
Oligonucleotide Reversed-Phase Columns
Ion pairing reversed-phase (IPRP) chromatography is the method of choice for synthetic oligonucleotide characterization and impurities analysis. The method helps scientists resolve synthesis and process-related impurities better than any other chromatographic technique, including anion exchange (AEX), HILIC, and SEC. The technique is not without its challenges. Multiple factors can directly affect separation performance and reproducibility, including the purity and stability of ion-pairing reagents, non-specific adsorption to chromatographic hardware, and batch-to-batch variations in sorbent. IPRP relies on high pH and temperature conditions for optimal separations, requiring column materials to have high pH and temperature tolerance.
Gain confidence in your results using Waters Oligonucleotide Reversed Phase Columns that offer:
- Industry-leading pH and temperature stability with BEH C18 hybrid silica particle technology for exceptional resolution and column life.
- A dramatic reduction of non-specific adsorption (NSA) with MaxPeak Premier High-Performance Surfaces
- (HPS) Technology for maximum sensitivity, reproducibility, and productivity.
- Oligonucleotide-based QC testing to ensure lot-to-lot separations performance and reproducibility.
BEH (Ethylene Bridged Hybrid) Technology
Ethylene Bridged Hybrid (BEH) particle technology ensures maximum column performance and longer column lifetimes under all chromatographic conditions. The hybrid particle technology offers many advantages over conventional silica-based particles, including the ability to control silanol activity for better reproducibility, peak shape, and efficiency. The BEH particle is created from two high purity monomers: tetraethoxysilane (TEOS) and bis(triethoxysilyl) ethane (BTEE), which results in highly stable, pH resistant, and mechanically strong particles that set the performance standard for method development. BEH particle technology allows for seamless transfer from analytical to preparative separations.
Specifications
Chemistry | C18 |
Separation Mode | Reversed Phase |
Particle Substrate | Hybrid |
pH Range Min | 1 pH |
pH Range Max | 12 pH |
Endcapped | Yes |
Silanol Activity | Low |
Molecular Weight Range Min | 1000 |
Molecular Weight Range Max | 30000 |
Particle Shape | Spherical |
Particle Size | 1.7 µm |
Endfitting Type | Parker-style |
Pore Size | 130 Å |
QC Tested | Oligonucleotide |
Format | Column |
System | UHPLC, UPLC |
Particle Technology | BEH |
USP Classification | L1 |
Inner Diameter | 2.1 mm |
Length | 50 mm |
Carbon Load | 18% |
UNSPSC | 41115709 |
Application | Oligonucleotide, Gene Therapeutics |
Brand | ACQUITY UPLC |
Product Type | Columns |
Units per Package | 1 pk |
- Model: 186003949
- Manufactured by: Waters