Waters XBridge Premier Protein BEH C4 Column with eConnect Column Tag, 300 Å, 2.5 µm, 4.6 x 100 mm, 1/pk - 186010333RF

Waters XBridge Premier Protein BEH C4 300 Å 2.5 µm, 4.6 x 100 mm, 1/pk with eConnect Column Tag

Product Number: 186010333RF

The XBridge Premier Protein BEH C4 300 Å 2.5 µm, 4.6 x 100 mm columns are QC tested with the MassPREP Protein Mix standard (p/n: 186004900) and are optimized to separate proteins based on size, hydrophobicity and isoelectric point. If you compare to traditional C18 phases, the C4 ligand is less retentive and will minimize protein carryover, increase protein recovery, and improve peak capacity. This column is part of the MaxPeak Premier Class of Columns, which deliver the chromatographic performance expected from our particle technologies while increasing reproducibility, peak shape, and recovery for metal-sensitive analytes.

Note: The same column is also available with the MassPREP Protein Mix Standard at no additional cost (p/n: 176005114)

Suggested use: Low-level intact protein and subunit analysis, phosphorylated proteins. This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These NFC-enabled device-tagged columns were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on any HPLC, but its advantages are unlocked when used with the Waters Alliance iS and Alliance iS Bio System running Empower software.

Protein Columns

Protein Analyses and Lab Scale Purifications

The development and successful commercialization of protein-based biopharmaceuticals and diagnostic reagents frequently depends on the ability to adequately characterize these complex biomolecules. Waters columns and methods can help solve your protein separation and characterization challenges.

Waters technology utilizes reversed-phase, ion-exchange, hydrophilic-interaction, and size-exclusion chromatography modes for peptide separation applications such as the analysis and purification of synthetic peptides, the characterization of complex proteolytic digests, and bioanalysis. These orthogonal separation techniques help provide the critical characterization data and isolated material required to produce the next generation drug.

BEH (Ethylene Bridged Hybrid) Technology

Ethylene Bridged Hybrid (BEH) particle technology ensures maximum column performance and longer column lifetimes under all chromatographic conditions. The hybrid particle technology offers many advantages over conventional silica-based particles, including the ability to control silanol activity for better reproducibility, peak shape, and efficiency. The BEH particle is created from two high purity monomers: tetraethoxysilane (TEOS) and bis(triethoxysilyl) ethane (BTEE), which results in highly stable, pH resistant, and mechanically strong particles that set the performance standard for method development. BEH particle technology allows for seamless transfer from analytical to preparative separations.

Related Products:

• 186004900 - MassPREP Protein Standard Mix
• 186009125 - Humanized mAb Mass Check Standard
• 176004434 - QuanRecovery with MaxPeak HPS Vial Package (186009186) with pre-slit PTFE silicone cap and septum, 100/pk

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