Waters XBridge Premier Peptide BEH C18 Column, 130 Å, 2.5 µm, 2.1 X 100 mm with Premier VanGuard FIT, 1/pk - 186010701

Waters XBridge Premier Peptide BEH C18 Column, 130 Å, 2.5 µm, 2.1 X 100 mm with Premier VanGuard FIT, 1/pk

Part Number: 186010701

The XBridge Premier Peptide BEH C18 Column, 130 Å, 2.5 µm, 2.1 X 100 mm with Premier VanGuard FIT Peptide columns have been specially optimized and QC tested with cytochrome c digest standards (p/n: 186006371) for peptide separations. The small-pore (130Å) trifunctionally bonded BEH particle offers the widest usable pH range, superior low pH stability, and ultra-low column bleed to assay samples for proteomics, protein characterization, and peptide synthesis. This column is part of the MaxPeak Premier class of columns, which deliver the chromatographic performance expected from our particle technologies while increasing reproducibility, peak shape, and recovery for metal-sensitive analytes such as phosphorylated peptides. The unique column design includes a fully integrated and easily replaceable 5mm pre-column that serves to protect and extend the life of the full-length analytical column, but adds no dispersion and has no impact on the resolution of your analytical separations. VanGuard FIT Cartridge replacements contain the same qualified sorbent as the analytical column and can easily be replaced.

Suggested Use: Peptide Separations, Peptide Analysis

• Suited for the reversed-phase analysis of hydrophobic and hydrophilic peptides at low and high pH
• Outstanding peak capacity and peak shape in TFA or FA when compared to 100% silica based C18 columns
• Two pore sizes (130 Å and 300 Å) for a wide range of peptides
• Superior peak shape and reduced tailing for acidic peptides
• Enhanced recovery and sensitivity for acidic peptides
• Available in OBD preparative 3.5 and 5 µm particles for seamless scalability and enhanced peptide isolation and purification

BEH (Ethylene Bridged Hybrid) Technology

Ethylene Bridged Hybrid (BEH) particle technology ensures maximum column performance and longer column lifetimes under all chromatographic conditions. The hybrid particle technology offers many advantages over conventional silica-based particles, including the ability to control silanol activity for better reproducibility, peak shape, and efficiency. The BEH particle is created from two high purity monomers: tetraethoxysilane (TEOS) and bis(triethoxysilyl) ethane (BTEE), which results in highly stable, pH resistant, and mechanically strong particles that set the performance standard for method development. BEH particle technology allows for seamless transfer from analytical to preparative separations.

The advantages of BEH columns include:

• Rugged hybrid organic/inorganic base particle
• Improved peak shape for bases-low silanol activity
• Wider operating pH range for method development flexibly
• Mechanical strength for maximum column efficiency at UHPLC operating pressures
• Available Particle Sizes: 1.7, 2.5, 3.5, 5, and 10 µm
• Column Chemistries: C18, C18 AX, Shield RP18, C8, Phenyl, Amide, Z-HILIC, HILIC

Related Products:

• 186010708 - ACQUITY Premier Peptide BEH C18 VanGuard FIT Cartridge, 1.7 µm, 2.1 X 5 mm, 3/pk
• 186002337 - MassPREP Peptide Mixture
• 186006371 - Cytochrome C Digestion Standard
• 186009126 - mAb Tryptic Digestion Standard
• 186010706 - XBridge Premier Peptide BEH C18 VanGuard FIT Cartridge, 2.5 µm, 2.1 X 5 mm, 3/pk
• 176005311 - PeptideWorks Tryptic Protein Digestion Start-Up Kit
• 186010109 - RapiZyme Trypsin, MS Grade, 4/pk with Buffer

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