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Waters 2414 Refractive Index (RI) Detector for Alliance HPLC Systems - 186241401

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2414 Refractive Index (RI) Detector

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Waters® 2414 Refractive Index (RI) Detector for Alliance® HPLC Systems

Product Number: 186241401

Reliable, repeatable analysis of non-absorbing components

Designed to provide scientists with the highest sensitivity, stability, and reproducibility, the 2414 Refractive Index (RI) Detector is the ideal solution for the analysis and quantification of components with limited or no UV absorption, such as alcohols, sugars, saccharides, fatty acids, and polymers.

With the 2414 RI Detector, you can detect components without UV chromophores, achieve highly reproducible detection with a wide linear dynamic range, and attain stable thermal control with temperature equilibration of incoming solvent and a thermally isolated optics bench. The 2414 RI Detector completes a total sample-centric solution for many applications, including polymer analysis, quality control (QC) applications, preparative analysis, analytical analysis, and food applications.

Overview

  • Achieve unattended operation and simplify methods development with auto-zero, auto-purge, and LED-displayed diagnostic programming
  • Support sustainability goals with a convenient recycling valve to conserve mobile-phase
  • Get faster warm-up times and baseline stability with improved temperature controls
  • Support advanced fraction collection routines with necessary external output
  • Control columns from 30–150 °C for a broad range of applications with control of an external column heater

Recommended Use: To analyze and quantify components with limited or no UV absorption.

Accurate quantification in quality control

Many quality control applications are challenged by the need for accurate and repeatable quantification of components that do not have a UV chromophore. The wide, repeatable measurement range offered by the 2414 RI Detector makes it an excellent choice for such QC applications, enabling reliable and repeatable component quantification.

Of the five recognized food sugars (fructose, glucose, sucrose, maltose, and lactose), the first three are the most important in fruit juice analysis. These sugars are quickly and easily analyzed on a system utilizing the 2414 RI Detector, the Alliance HPLC System, and an XBridge Amide XP Column.

Of the five recognized food sugars (fructose, glucose, sucrose, maltose, and lactose), the first three are the most important in fruit juice analysis. These sugars are quickly and easily analyzed on a system utilizing the 2414 RI Detector, the Alliance HPLC System, and an XBridge Amide XP Column.

Highly repeatable polymer analysis

Polymer analysis by gel permeation chromatography (GPC) requires a highly repeatable system to obtain an accurate measurement of the molecular weight of the polymers. The 2414 RI Detector is designed to manage these stringent requirements for the chromatographic analysis of polymers. The thermal design of this detector delivers low noise and drift performance for accurate polymer measurements, even at lower concentrations. Combined with the highly repeatable solvent delivery of the Alliance HPLC System and the powerful data processing and reporting capabilities provided by the Empower Software GPC option, this system offers the ultimate solution for accurately and reproducibly determining the molecular weight distribution of a polymer.

Temperature stability and high sensitivity in the 2414 RI Detector allow for the analysis of polymers at low concentration.

Temperature stability and high sensitivity in the 2414 RI Detector allow for the analysis of polymers at low concentration.

Innovative thermodynamic managementSample flow enters the counter-current heat exchanger traveling through an internal capillary into the flow cell. When exiting the flow cell, it flows through a larger I.D. tubing that surrounds the inlet capillary. This thermally equilibrates the incoming sample-flow temperature, and dramatically minimizes drift.

Waters innovative counter-current heat exchanger quickly equilibrates temperature differences between the solvent stream entering and exiting the flow cell. This non-traditional heat exchanger prevents fluctuations in viscosity and refractive index, other than those changes caused by the sample itself. Consequently, the reproducibility is significantly enhanced with an optional, detector-controlled column heater, capable of stabilizing mobile phase viscosity prior to entering the detector.

The counter-current heat exchanger provides:

  • Improved temperature control
  • Faster warm-up times, baseline stability
  • Low baseline noise and drift

Sample flow enters the counter-current heat exchanger traveling through an internal capillary into the flow cell. When exiting the flow cell, it flows through a larger I.D. tubing that surrounds the inlet capillary. This thermally equilibrates the incoming sample-flow temperature, and dramatically minimizes drift.

Flexible implementation

The 2414 RI Detector delivers a streamlined user experience, offering the flexibility to use it stand-alone or as part of any of Waters HPLC system solutions. Advanced fraction collection routines and control of external HPLC column ovens is supported by full module control in Empower Software. 

You can achieve simplified operation with:

  • Front panel control
  • Auto-purge that conveniently fills the reference flow cell with fresh mobile phase
  • User-accessible diagnostic functions, including automated noise and drift tests
  • Ease of use with simplified optics bench alignment, so routine maintenance is not required

Specifications:

Operating Specifications¹
Refractive index range 1.00 to 1.75 RIU
Measurement range 5.0 x 10-4 RIU to 7.0 x 10-9 RIU
Linear dynamic range ≤5.0% over ±5.0 x 10-4 RIU
Noise2 ±1.5 x 10-9 RIU (2 s FTC Hamming 1.0 mL/min, 100% H2O)
±3.0 x 10-9 RIU 410/2410 emulation mode (1 s FTC (RC) 1.0 mL/min, 100% H2O)
Drift 2.0 x 10-7 RIU/hour
Filter time constant 0.0 to 5.0 s (Hamming)
0.0 to 10.0 s (RC)
Compatible flow rate range 0.1 to 10.0 mL/min
Attenuation settings 1 to 500 x 10-6 RIU
1 to 1024 maximum in emulation mode
Temperature control Internal oven: 30 to 55 °C (86 to 131 °F), ±0.5 °C, settable 1 °C increments
One external column heater, steel: ambient to 150 °C (302 °F), ±1.0 °C, settable 1 °C increments
Optical Component Specifications
Flow cell Fused quartz
Flow cell volume 10 μL
Flow cell pressure limit ≤100 psi
Light source LED 870 nm
Wetted materials 316 stainless-steel, PTFE, PEEK, quartz
Electrical Specifications
Power requirements 100 to 240 VAC
Line frequency 50 to 60 Hz
Power consumption 145 VA (nominal)
Inputs Seven event inputs
Outputs Three outputs (2 analog and 1 event)
Physical / Environmental Specifications
Dimensions Width: 34.3 cm (13.5 inches)
Height: 20.8 cm (8.2 inches)
Depth: 61.0 cm (24.0 inches)
Weight: 16.3 kg (36.0 pounds)
Operating temperature range 15 to 40 °C (59 to 104 °F)
Operating humidity range 20% to 80%, non-condensing
Audible noise <58 dBA
Ordering Information
Part Title Part Number
2414 Refractive Index Detector for Alliance HPLC Systems 186241401
2414 Refractive Index Detector for ACQUITY Arc Systems 176017008
Column heating compartment WAT038040
  1.  All performance specifications are measured following a warm-up period of 2 hours with ambient Δ T ≤±2.0 °C/hour
  2. ASTM E1303-95

  • Model: 186241401
  • Manufactured by: Waters