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3465 Electrochemical Detector for LC Systems
Please contact our Sales Team for a quote.
Sensitivity, reliability, and simplicity combined
The Waters 3465 Electrochemical Detector combines sensitivity, reliability, and simplicity for electrochemical detection. Its versatile detection modes are ideally suited for analyzing a wide range of compounds.
The 3465 Electrochemical Detector’s multiple flow cell configuration combines working and reference electrode designs to minimize cost and enhance flexibility. The flow cell accommodates several reference electrodes: ISAAC; traditional salt-bridge design, which limits air bubble formation; and Hy-Ref, which is ideal for carbohydrate analysis and is compatible with extreme mobile phases. The detector is designed for ultra-trace analysis and is compatible with both analytical and microbore scale applications.
Overview
- Selectivity for a wide range of applications such as catecholamines, aminoglycosides, carbohydrates, neurotransmitters, phenols, and thiols with multiple flow cell, electrode, and detection mode options
- Sensitivity for ultra-trace analysis to detect concentrations as low as 10 ρmol/L and as high as µmol/L
- Simplicity of operation plus versatility with Empower control and use with the Waters liquid chromatography (LC) portfolio
- Reliability backed by Waters service and application support and 21CFR Part 11 compliance readiness
Recommended Use: For ultra-trace analysis compatible with both analytical and microbore scale applications.
Advanced sensitivity
- Achieve ultra-trace analysis
- Eliminate matrix interferences with an Advanced Digital Filter
- Improve thermal operating stability with Faraday-shielded oven
Overlay of chromatograms of 0.008 mg/mL kanamycin sulfate and 0.020 mg/mL amikacin obtained in a USP specified mobile phase (n=4).
Flexible for your requirements
The 3465 is controlled by industry-leading Empower Chromatography Data System (CDS) and compatible with the Arc HPLC, Alliance, ACQUITY Arc, and ACQUITY UPLC H-Class Systems, 1515 isocratic and gradient Empower CDS-based systems, Breeze, and QS-based systems when configured with an eSat/IN analog/Digital convertor. There are two configurations of the 3465 ECD: one supports control of a single flow cell and the second supports the independent control of dual flow cells.
Specifications:
| OPERATING SPECIFICATIONS | |
| Operating modes | Direct Current (DC), DC, PULSE, PULSE 2, SCAN (DC, PULSE, and PULSE 2 are available and supported under Empower CDS control) |
| Other modes | CONFIG, DIAG, and SERVICE |
| Detector configurations | Single Flow Cell (SCC) (p/n: 725000673) or Dual Flow Cell (DCC) (p/n: 725000686) |
| Autozero | Triggered by keyboard, rear panel TTL, or remote PC control (LAN) |
| Max. current compensation (Autozero) | 25 nA - 2.5 mA in DC and PULSE mode dependent on range setting |
| Offset | +50% to -50% of max. output voltage, 5% steps |
| PC control | Parametric control and data-acquisition via LAN port (USB service port) |
| Acquisition control | .Net Driver for Empower 3, leveraging existing Empower 3 ICF Support layer |
| Analog signal output (DAC) | -1 to +1 V full scale (via 16-bit D/A converter) |
| Analog output (I/E) | -2.5 to +2.5 V full scale (unprocessed I/E converter signal) |
| Digital I/O connectors (rear panel) | 2x relay, 5x TTL outputs (CMOS 3.3V logic), 13 TTL inputs (programmable), 1x GND |
| Programmable I/O functionality | Cell on, Cell off, Autozero, Start, Overload, Relay, Auxiliary |
| Integrated flow cell and column heating compartment | +7 °C above ambient to 60 °C, accuracy 0.5 °C, stability 0.1 °C; accommodates column and flow cell(s) |
| Flow cell | Confined wall-jet design - VT03, Sencell, and thin-layer design Flexcell |
| VT03 | Flow cell (originally available with 2465 ECD); available with 2- and 3-mm working electrode diameter |
| Sencell | Tool-free adjustable spacer; multiple working and reference electrodes |
| Flexcell | Replaceable working electrode, (GC, Pt, Au, Ag, BDD) stainless steel auxiliary electrode |
| DC MODE | |
| Range | 10 pA - 200 µA in 1, 2, or 5 increments |
| Filter (ADF) | 10 - 0.001 Hz in 1, 2, or 5 increments; RAW and OFF: For unprocessed data |
| Potential (Ec) | -2.5 V to +2.5 V with 10 mV increments |
| Data rate | 1 - 100 Hz in 1, 2, or 5 increments, dependent on filter setting |
| Noise | <2 pA with dummy cell (load of 300 MΩ/470 pF) in 1 nA range, filter off, Ec +800 mV, and temp. of 35 °C |
| PULSE MODE | |
| Range | 10 nA - 200 µA in 1, 2, or 5 increments |
| Filter (ADF) | 0.5 - 0.001 Hz in 1, 2, or 5 increments; OFF: For unprocessed data |
| Potential (Ec) | -2.5 V to +2.5 V with 10 mV increments |
| Data rate | 1/(pulse duration) Hz |
| Waveform | Max five potential steps |
| Pulse times (t1-t2) | t1: 100 ms - 2000 ms; t2, t3, t4, and t5: 0 - 2000 ms in 10 ms increments |
| Sampling times (ts) | 20 ms – [t1 – 60] ms |
| PULSE MODE 2 | |
| Range | 10 nA - 200 µA in 1, 2, or 5 increments |
| Filter (ADF) | 0.5 - 0.01 Hz in 1, 2, or 5 increments; OFF: for unprocessed data |
| Potential (Ec) | -2.5 V to +2.5 V with 10 mV increments |
| Data rate | 1/(pulse duration) Hz |
| Waveform | Free programmable multi-step waveform with up to 30 time-potential (t, E) coordinates and max pulse duration of 4 s; time points in 10-ms increments |
| Sampling time | Sampling interval is freely programmable, defined by Begin and End markers |
| SCAN MODE | |
| Range | 10 nA - 200 µA in 1, 2, or 5 increments |
| Potential (Ec) | -2.5 V to +2.5 V with 10 mV increments |
| Data rate | 1 Hz |
| Scan rate | 1 - 100 mV/s in 1, 2, or 5 increments |
| Cycle | Half, Full, or Continuous |
| ENVIRONMENTAL SPECIFICATIONS | |
| Usage | For indoor use only |
| Solvent storage | <2000 m |
| Operating temp. range | 10 – 35 °C |
| Operating humidity range | 20% to 80% RH |
| ELECTRICAL SPECIFICATIONS | |
| Voltage range | 100-240 VAC autosensing |
| Voltage fluctuation | Input voltage of 100-240 VAC +/- 10%† |
| Line frequency | 50-60 Hz |
| Power requirements | 260 VA |
| † Derating may be needed under low input voltages | |
| PHYSICAL SPECIFICATIONS | |
| Weight | 14.4 Kg (32 lbs.) without flow cell or column (SSC version) |
| Width | 22 cm (8.7 “) |
| Height | 44 cm (17.3 “) |
| Depth | 43 cm (16.9 “) |
| Safety and EMC compliance | CE (CB Scheme), UL, and CSA (cMETus approval) |
| Audible noise | 45 dBA (detector on and the door closed) |
| ORDERING INFORMATION | |
| Description | Part Number |
| 3465 Electrochemical Detector | 725000673 |
| 3465 Dual Flow Cell Electrochemical Detector | 725000686 |
| Flowcell 2-mm Glassy Carbon WE/Salt Bridge Ref (used for qualification) | 700013052 |
| Flowcell Kit, 2-mm Glassy Carbon WE/ISAAC REF, VT03 (used for qualification) | 205004215 |
- Model: 3465 Electrochemical Detector
- Manufactured by: Waters
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