Waters ACQUITY UPLC and ACQUITY Premier Fluorescence (FLR) Detectors

ACQUITY UPLC and ACQUITY Premier Fluorescence (FLR) Detectors

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Unrivaled sensitivity and selectivity in UPLC detection

The superior, high-efficiency separations of UHPLC and UPLC technology require high sensitivity and selectivity as well as high-performance detection techniques to effectively acquire and quantitate chromatographic data.

The ACQUITY UPLC and ACQUITY Premier Fluorescence (FLR) detectors are high-sensitivity, multi-channel fluorescence detectors further optimized for use with the ACQUITY UPLC PLUS Series and ACQUITY Premier Systems, respectively. These detectors boast features like innovative flow cell design, low-noise electronics and support for high-speed data rates that bring unrivaled sensitivity and selectivity to UPLC/UHPLC separations.

Overview

• Achieve accurate and reproducible integration with effective integration algorithms, sampling rates of up to 80 Hz, and independent optimization of filtering constants
• Quickly determine wavelength maxima with flexible 2D and 3D scanning capabilities
• Ensure confidence in performance with an intuitive software interface and diagnostic tools
• Achieve enhanced sensitivity from a low volume (<2 μL), axially illuminated flow cell design, allowing for better light absorption
• Improve signal-to-noise performance with advanced optical design to maximize light throughput and reduce light scatter
• Completely characterize the narrow, sharp peaks typical of UHPLC, and UPLC separations with high-speed data rates (up to 80 Hz) and independent optimization of filter time constants
• Significantly reduce the time to develop fluorescence detection methods by quickly determining wavelength maxima with multiple detection modes - 2D, 3D, and on-the-fly spectral scanning 

Recommended Use: For analyzing polynuclear aromatic hydrocarbons (PAHs), drugs of abuse, vitamins, and any component with chemiluminescent properties.

Meeting today’s analytical challenges

The primary advantage of fluorescence detection over other techniques is the intrinsic sensitivity offered by fluorescence coupled with outstanding selectivity – as relatively few molecules fluoresce naturally.

The overall design of the ACQUITY UPLC and ACQUITY Premier FLR Detectors achieve both the high sensitivity and selectivity needed by laboratories to ensure that analytes requiring the lowest limits of detection are accurately detected and quantitated, whether fluorescent naturally or derivatized with a fluorescent tag.

High sensitivity analyses require high-sensitivity detection. FLR detector optics results in the efficient detection of Anthracene (12 fg on-column) at levels as low as 3.2 fg.

Fast method optimization

Developing efficient methods requires the determination of wavelength maxima for each compound in a sample. As optimum excitation (Ex) and emission (Em) wavelengths vary from compound to compound, the detector’s flexible 2D and 3D spectral acquisition modes enable users to quickly and accurately determine optimal detector wavelength settings.

As an added benefit, ACQUITY UPLC and ACQUITY Premier FLR Detector users can generate on-the-fly or static spectral scans – enabling quick optimization of methods and on-demand peak confirmation when compared against stored spectra.

Characterize unknowns via the Spectrum λ-λ detection mode. Both excitation and emission wavelengths can be plotted on the same 3D contour plot for easy determination of optimum wavelength maxima.

Intuitive, easy-to-use software interface

ACQUITY UPLC and ACQUITY Premier FLR Detectors are equipped with a customizable instrument console for both Empower and MassLynx software, which provides users with more control. Interactions historically done through an instrument keypad (instrument setup, status monitoring, and system diagnostics) are now readily accessible through an intuitive, easy-to-learn software interface.

The console interface uses a simple navigational approach for easy system implementation and instrument usability and allows for quick and easy access to critical instrument parameters – enabling the FLR Detector to be easily controlled, monitored, and diagnosed.

ACQUITY UPLC FLR Detector console.

Specifications: