1525 Binary HPLC Pump
Standard analytical flow rates to Semi-preparative flow rates
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Waters® 1500 Series HPLC Pumps have been designed to meet all of your flow range needs, from low flow to preparatory. Exceptional flow rate across all ranges provides you with precision and accuracy, delivering the chromatographic reproducibility that you need to fully automate all your processes. With exceptional solvent blending and pulse-free delivery, the Waters 1500 Series HPLC Pumps can meet your most sensitive application challenges.
The 1525 and 1525 with EF heads are integrated, high-pressure, binary HPLC pumps that feature on-board pulse dampening and efficient mixing. Their pulse-free solvent flow, from analytical to semi-preparative flow rates, makes them ideal for use with highly sensitive detectors.
Designed for use with 2 to 4.6 mm I.D. columns in routine LC and LC/MS applications, these pumps have a programmable flow-rate range of 0.00 to 10.00 mL/min in 0.01 mL increments.
Optional features:
- Extended Flow (EF) heads for extended flow rate range up to 22.50 mL/min for use with 10 to 19 mm I.D. columns
- 1500 Series Column Heater with a temperature range of 20 to 60 °C to maintain accurate, consistent column temperature
- Integral vacuum degasser to remove dissolved gases from the mobile phase, improving performance and reliability
- Automated seal wash helps extend the primary seal life for reliable operation
- FlexInject manual dual injector module for both analytical and semi-preparative injections
1500 Series HPLC Pumps
Pump Name | Flow Rate |
1525 Micro Binary HPLC Pump | Low flow rates |
1525 Binary HPLC Pump | Standard analytical flow rates to Semi-preparative flow rates |
1525 with Extended Flow Heads | Standard analytical flow rates to Semi-preparative flow rates |
1515 Isocratic HPLC Pump | Standard analytical flow rates to Semi-preparative flow rates |
Specifications:
OPERATING SPECIFICATIONS | |
Number of eluents | 1515: One 1525: One or two |
Programmable flow rate range | 0.00 to 10.00 mL/min in 0.01 mL increments |
Flow precision | ≤ 0.1% RSD or 2 sec SD, six replicates, based on retention time or volumetric measures, at 1.0 mL/min, 1000 to 2000 psi backpressure, PQ test (each pump individually) |
Flow accuracy | ± 1.0% of setting at 1.0 mL/min or 30.0 μL/min, whichever is greater, using degassed methanol, 1000 to 2000 psi backpressure (each pump individually) |
Maximum operating pressure | 6000 psi (41,370 kPa, 401 bar) |
Operating pressure limits | Software programmable high and low pressure limits; user selectable in psi, kPa, bar < |
Delay volume (1525) | 200 μL (with one GM 150 mixer) |
Gradient compositional accuracy (1525) | ± 0.5% of setting at 1 mL/min and 1000 psi backpressure (methanol:methanol with propylparaben) with one GM 150 mixer |
Gradient compositional precision (1525) | < 0.5% RSD of setting at 1.0 mL/min and 1000 psi back pressure (methanol:methanol with propylparaben) with one GM 150 mixer (based on six replicates of compositional accuracy) |
Pressure ripple (one pump) | ≤ 2.0% at 1.0 mL/min, degassed methanol, at 1000 psi backpressure |
Gradient ripple | < 1.5% normalized to full scale between 10% to 90%, 1.0 mL/min, and 1000 psi backpressure (methanol:methanol with propylparaben with one GM 150 mixer) |
1525μ Binary HPLC Pump | |
OPERATING SPECIFICATIONS | |
Programmable flow rate | 0.00 to 5.00 mL/min, in 0.01 mL/min increments |
Flow precision | 2 sec SD, six replicates based on retention time or volumetric measures at 0.2 mL/min, 1000 to 2000 psi back pressure, PQ test (each pump individually) |
Flow accuracy | 2.0% of setting at 0.1 mL/min using degassed methanol at 1000 to 2000 psi backpressure (each pump individually) |
Maximum operating pressure | 6000 psi (41,370 kPa, 401 bars) |
Operating pressure limits | Software programmable high and low pressure limits; user selectable in psi, kPa, bar < |
Delay volume | 30 μL (without mixer), < 100 μL (with one 50 μL mixer) |
Gradient accuracy | < 1.0% of setting (typical) at 0.1 mL/min between 10% to 90%, and 1000 psi backpressure (methanol:methanol with propylparaben and one 50.0 μL mixer) |
Gradient precision | < 0.5% RSD (typical) between 10.0% to 90.0%, 0.1 mL/min, and 1000 psi backpressure (methanol:water with octanophenone and one 50.0 μL mixer) |
Pressure ripple (one pump) | < 2.0% at 0.5 mL/min, degassed methanol, at 1000 to 2000 psi backpressure |
Gradient ripple | < 1.5% normalized to full scale between 10.0% to 90.0%, 0.1 mL/min, and 1000 psi backpressure (methanol:methanol with propylparaben) with one 50.0 μL mixer |
INSTRUMENT CONTROL | |
Communications | Ethernet, IEEE-488 |
External control | Empower™ Breeze™ or MassLynx™ Software |
Event I/O | Back of instrument Detachable terminal strip |
PHYSICAL / ENVIRONMENTAL SPECIFICATIONS | |
Physical size (without bottle holder) | Height: 30.5 cm (12.0 in.) Depth: 61.0 cm (24.0 in.) Width: 43.0 cm (17.0 in.) |
Weight | 1515: 20.4 kg (45.0 lbs) 1525: 27.2 kg (60.0 lbs) |
Wetted surface material | 316 stainless steel, sapphire, reinforced fluorocarbon polymer seals, carbon-reinforced Tefzel < |
Acoustic noise | 70 dB(A) |
Operating temperature range | 4 to 40 °C |
Operating humidity range | 20% to 80%, non-condensing |
1500 Series Manual Injector (Applicable for 1515 and 1525) - Rheodyne 7725I Injector | |
OPERATING SPECIFICATIONS | |
Inject switch | Contact closure, pre-wired |
Mounting | Integral for 1500 Series HPLC pumps |
Sample holdup | Zero |
Flow during switching | Continuous, make-before-break |
Injection | Partial or full loop |
Loop size | 20 μL (standard) Changeable (5-, 50-, and 200-μL loop supplied) |
Wetted materials | 316 ss, ceramic, inert polymers |
Waters 1500 Series Column Heater | |
OPERATING SPECIFICATIONS | |
Set point temperature range | 20 to 60 °C; set ≤ 5 °C above ambient temperature |
Temperature accuracy | ± 0.80 °C |
Temperature precision | ± 0.25 °C |
Pre-column heating of mobile phase | ± 0.50 °C of column temperature up to 5.00 mL/min |
Pre-heat tube volume | Approximately 35 μL |
Column capacity | Up to four 7.8 mm x 300.0 mm without guard columns Two columns with guard columns |
PHYSICAL SPECIFICATIONS | |
Dimensions | Height: 43.0 cm (17.0 in.) Depth: 35.6 cm (14.0 in.) Width: 15.2 cm (6.0 in.) |
Weight | 5.9 kg (13.0 lbs) |
ELECTRICAL SPECIFICATIONS | |
Input voltage range | 120/240 VAC |
Input frequency range | 50/60 Hz |
INSTRUMENT CONTROL | |
Communications | Ethernet, IEEE - 488 |
- Model: 186001525
- Manufactured by: Waters