Description :  

Introduction

The EZgene^TM mycoplasma gDNA kit provides a fast and easy method for isolating gDNA from mycoplasma. The system utilizes the reversible nucleic acid-binding properties of Biomiga's ezBind membrane and the speed of spin column technology to yield high quality gDNA with the OD_260/280 ratio of 1.7-1.9. Purified DNA is ready for applications such as PCR, Southern Blotting, and Restriction Digestion.

Storage and Stability

All EZgene^TM mycoplasma gDNA Kit components are guaranteed for at least 12 months from the date of purchase when stored as follows:

• Reconstituted Protease K and store at -20°C.

• All other materials at room temperature (22-25°C).

Materials provided by user

• Tabletop microcentrifuge

• Sterile 1.5 mL centrifuge tubes

• Water bath

• Absolute ethanol

• PBS Buffer

Kit Contents

Before Starting

The kit is designed to be simple, fast, and reliable provided that all steps are followed diligently. Please read the entire booklet and get all necessary supplies and equipments.

Important

• Reconstitute Protease K with 110 μL (GD3211-00), 1.3 mL (GD3211-01), or 5 x 1.3 mL (GD3211-02) Elution Buffer and vortex the vial briefly before use. Aliquot and store vials of reconstituted Protease K at -20°C.
• Dilute DNA Wash Buffer with absolute ethanol as follows:       

Add 8 mL (GD3211-00) or 60 mL (GD3211-01) or 96 mL (GD3211-02) of absolute ethanol to each bottle. The final concentration is 70%.

• Under cool ambient conditions, precipitates may form in Buffer LY. In case of such an event, heat the bottle at 37°C to dissolve before use.

Determination of Yield and Quality

The total DNA yield can be determined by a spectrophotometer using deionized water, Tris-HCl Buffer, or Elution Buffer as a blank. Dilute the DNA in TE Buffer and calculate the concentration using the following equation:

[DNA] = (Absorbance ₂₆₀) x (0.05 μg/μL) x (Dilution Factor)

The quality of DNA can be assessed by measuring absorbance at both 260 nm and at 280 nm. A _{260/ 280} ratio of 1.7-1.9 corresponds to 85%-95% purity.

Limited Use and Warranty

This product is intended for in vitro research use only. Not for use in human. 

This product is warranted to perform as described in its labeling and in Biomiga's literature when used in accordance with instructions. No other warranties of any kind, express or implied, including, without limitation, implied warranties of merchantability or fitness for a particular purpose, are provided by Biomiga. Biomiga's sole obligation and purchaser's exclusive remedy for breach of this warranty shall be, at the option of Biomiga, to replace the products, Biomiga shall have no liability for any direct, indirect, consequential, or incidental damage arising out of the use, the results of use, or the inability to use it product.

Operating Protocol

For technical support or learn more product information, please contact us at (858) 603-3219 or visit our website at http://www.biomiga.com/.