Dr. Maisch Exsil 100 SAX, 5 µm, 100 Å, 1 g bulk material for HPLC Columns - 5136529.0001

ExSil® 100

Dr. Maisch Exsil 100 SAX, 5µm, 100Å, 1g bulk material for HPLC Columns

Product Number: 5136529.0001

Exsil 100, the standard material, is supplied in a range of particle sizes and bonded phases to cover a wide range of applications. Exsil 80 is a higher surface area, complementary range of materials.

SAX (Strong Anion Exchange) columns in HPLC are used to separate anionic compounds based on their charge. They are typically silica-based columns with a positively charged quaternary ammonium functional group chemically bonded to the silica surface, allowing them to interact with negatively charged molecules. These columns are compatible with aqueous and low pH mobile phases and are particularly useful for analysing smaller organic molecules like nucleotides and organic acids, as well as larger biomolecules like proteins.

• Stationary Phase: SAX columns contain a silica-based material with positively charged quaternary ammonium functional groups attached to the surface.
• Separation: Anionic compounds in the sample mixture will interact with the positively charged stationary phase based on their charge and hydrophobicity.
• Elution: Anionic compounds are eluted from the column by changing the mobile phase conditions, such as increasing ionic strength or changing the pH, which disrupts the interaction between the analyte and the stationary phase.
• Applications: SAX columns are widely used in pharmaceutical analysis, environmental analysis, and analysis of biomolecules. Separation of nucleotides and organic acids, Analysis of oligonucleotides and proteins, Purification of biomolecules, and Separation of sulfated disaccharides.
• Exsil 100: Columns with a pore size of 100 Å Angstroms (10 nm)