Intrada SEC silica-diol LC and LC-MS Column
Intrada SEC 3um, Diol-Silica for SEC Semi-Preparative HPLC Column, 250mm x 20mm
Imtakt Product Number: WSEQ6
Column Specifications:
Base Material: | Silica |
Modification: | Diol |
Quantity: | 1 Piece |
Manufacturer's Chemistry Name: | Diol |
Matrix: | Silica |
Inside diameter: | 20 mm |
Length: | 250 mm |
Particle Size (Microns): | 3µm |
Brand name: | Intrada |
Particle morphology: | spherical, porous |
Particle size: | 3 μm |
Pressure stability: | 250 bar |
Mode: | Diol phase |
Pore size: | 130 Å |
Technology: | HPLC |
USP Class: | L20 |
pH: | 1-8 |
World's first silica-diol SEC column designed to work with LC and LC-MS compatible volatile salts
- Compatible with hydrophilic polymers like proteins (GFC)
- Compatible with hydrophobic polymers like polystyrene (GPC)
- Uses volatile solvents as standard eluents
- Extended lifetime under pH 1-8
An Innovative Size Exclusion Spherical Porous Silica, Analytical Column, Compatible with GFC/GPC
Intrada SEC is a wide pore (300A), fully porous silica material with a bonded diol substitution stationary phase. This innovative technology overcomes some traditional problems with diol-silica columns, such as the need for use of high concentrations of non-volatile salts in the eluent and poor lifetime due to undesired residual silanols effect. The next generation Intrada SEC column allows both aqueous durability like a polymer-based SEC column and the mechanical strength of a silica-based SEC column. This column can be used with volatile salts, such as 100mM ammonium formate, as the eluent for aqueous polymer analytes, making it compatible with ELSD or MS detection.
Another benefit of the technology behind the Intrada SEC column is that it can be used for both aqueous GFC (proteins, polysaccharides and nucleic acids etc.) and non-aqueous GPC (polystylene, PVAc etc.), making it a real unified "SEC" column.
Ability to use volatile salt eluents may change SEC history
Traditionally, it has been required to use 100mM phosphate buffer + 300mM NaCl as an eluent for diol- silica SEC columns due to an undesired ionic effect, caused by exposed residual surface silanols. But use of high concentrations of inorganic salt is too harsh for use with most LC systems and also their non-volatile nature makes them incompatible for use with an MS detector. The innovative Intrada SEC column with a novel diol substitution technology allows for the use of 100mM ammonium formate instead, which is a volatile eluent that is compatible with MS and ELSD detection. This truly revolutionary design may change the history of SEC.
Range of Intrada SEC Diol-Silica Columns
3µm Column, Pressure limits of up to: 20MPa, 250 bar, 3,000 psi
Column Length (mm) | Analytical | Semi-prep | Preparative | |
4.6 mm I.D. | 6.0 mm I.D. | 10.0 mm I.D. | 20.0 mm I.D. | |
250 | WSE06 | WSEP6 | WSEQ6 | |
Guard Cartridges | ||||
4.6mm I.D. | GCWSES | |||
10-20mm I.D. | GCWSEM | |||
Guard Holders | ||||
4.6mm I.D. | GCH11S | |||
10-20mm I.D. | GCH12M |
Additional Imtakt Columns Available
- Model: WSEQ6
- Manufactured by: Imtakt