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Imtakt Unison US-Amino 1mm ID, 50mm Length, 5um,Aminopropyl Silica - USA12

Price: £337.68


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Unison US-Amino 5μm Column, Durable against water elusion

Unison US-Amino (Aminopropyl) 5μm, 50mm x 1mm, 130Å Analytical HPLC Column, Pressure Limits of up to : 20MPa, 250 bar, 3,500 psi

Imtakt Product Number: USA12

Column Specifications:

Base Material: Silica
Quantity: 1 piece
Chemistry: NH2 (Amino)
Manufacturer Chemistry Name:  US-Amino
Endcapping:  -
Matrix: Silica
Brand: Imtakt Unison 
Internal Diameter: 1 mm 
Length: 50 mm 
Particle Morphology: spherical, porous
Particle Size: 5 μm
Pressure Stability: up to 250 bar
Pore Size:  130 Å
Technology: HPLC
USP Class: L8

High quality long-life Amino column for carbohydrate or Hilic separations.

The Unison Family of columns provide a wide range of high quality reverse phase and normal phase columns. The quality of our material provides high efficiency with low backpressure and 100% aqueous stability. Peak shape is also improved with the use of our polymeric end-capping technology. The Unison series of columns are available with C18, C8, Phenyl, Amino and Silica stationary phases to meet a wide range of chromatographic needs.

  • Pure spherical porous silica 5µm particle Aminopropyl phase
  • High efficiency 5µ columns with mono-dispersed packing for low backpressure
  • Normal phase aminopropyl material ideal for analysis of sugars and carbohydrates
  • World class reproducibility
  • HT and UP hardware able to operate in pressures up to 1000 bar
  • High efficiency and available short column dimensions are ideal for high throughput applications
  • Aqueous to non-aqueous Normal Phase separation
  • LC-MS applicable
  • Unique ligand binding strategy provides dramatically longer lifetimes and lower bleed compared to other amino columns

Aqueous durable silica-based aminopropyl columns have been used for a long time as a normal phase column for carbohydrate separation. However, these aminopropyl columns have a fatal flaw: "column bleeding" or the rapid deterioration in retention as a result of ligand desorption under aqueous elution.

Unison UK-Amino offers a different approach from conventional columns: high durability against aqueous eluent. As the above chromatogram demonstrates, conventional columns experience a significant decline in retention when an aqueous mobile phase elutes through the column. UK-Amino, on the other hand, does not show any change in separation or retention. This is a significant development in the history of aminopropyl columns.

UK-Amino's design not only provides analytical power, but the 3um particle high-resolution column has other benefits, including the minimization of LC-MS and LC-ELSD noise levels. UK-Amino can be applied to aqueous normal phase conditions, and separation optimization is possible while comparing to ODS columns using reversed-phase mode. One can expect significant results from this normal phase column of UK-Amino.

Range of Unison US-Amino 5µm Columns, Product Numbers:

Column Length (mm) Analytical Semi-prep Prep
1.0 mm I.D. 1.5 mm I.D. 2.0 mm I.D. 3.0 mm I.D. 4.0 mm I.D. 4.6 mm I.D. 6.0 mm I.D. 10.0 mm I.D. 20.0 mm I.D. 28.0 mm I.D.
5 µm Columns, Pressure Limits of up to : 20MPa, 250 bar, 3,500 psi
10     USA20 USA30   USA00        
20     USA29 USA39   USA09        
30 USA11 USA71 USA21 USA31   USA01 USA61 USAP1    
50 USA12 USA72 USA22 USA32   USA02 USA62 USAP2 USAQ2  
75 USA13 USA73 USA23 USA33   USA03 USA63 USAP3    
100 USA14 USA74 USA24 USA34   USA04 USA64 USAP4 USAQ4  
150 USA15 USA75 USA25 USA35 USA45 USA05 USA65 USAP5 USAQ5  
250 USA16 USA76 USA26 USA36 USA46 USA06 USA66 USAP6 USAQ6 USAR6

Column Guard System for Unison US-Amino:

Product Code Product Name Product Description
GCUSAC Guard Cartridge US-Amino 5um,Aminopropyl Silica (5x1mm), 3pcs
GCUSAM Guard Cartridge US-Amino 5um,Aminopropyl Silica (10x8mm), 2pcs
GCUSAS Guard Cartridge US-Amino 5um,Aminopropyl Silica (5x2mm), 3pcs
GCH01S Guard Holder Guard Holder for 1-6mmI.D. separation columns
GCH02M Guard Holder Guard Holder for 10-20mmI.D. separation columns
GCH11S Guard Holder Guard Holder for 4.6mm column, enclosed an U-Shape column coupler
GCH12M Guard Holder Guard Holder for 10-20mm column, enclosed an U-Shape column coupler

All stationary phases can also be made in the following internal diameters, please contact our Sales Team with your specificatons: Nano: 0.02mm, 0075mm | Capillary: 0.1mm, 0.3mm, 0.5mm | Semi-Prep: 20mm, 28mm.

This Column is also Available in 3μm!

Additional Imtakt Columns Available


  • Model: USA12
  • Manufactured by: Imtakt

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