Miles Scientific Analtech Silica Gel G (Gypsum) Prep TLC Plates, Prep-scored, UNIPLATE-T Taper, Glass-backed, 20 x 20 cm, 25/Pk - P80003

Glass-backed Prep Special TLC Plates

Miles Scientific Analtech Brand Silica Gel G Prep Thin Layer Chromatography (TLC) Plates, UNIPLATE-T Taper Plate, Prep-scored - Glass-backed - 20 x 20 cm - 25 plates per box

Product Number: P80003

Weight: 15.10 LBS

• G - (Gypsum) Calcium Sulfate Hemihydrate binder

Tapered Uniplate-T™

The UNIPLATE-T Taper Plate, invented and patented by Analtech, is a unique preparative TLC plate that provides unusually high resolution for a preparative layer. It has a wedge-shaped adsorbent layer, progressing from 300 microns (bottom) to 1700 microns (top). Below the silica layer is a 700-micron-thick preadsorbent zone provided for easier sample application and higher sample resolution (see figure below). Sample concentration prior to separation occurs in this preadsorbent zone.

The tapered adsorbent layer causes low Rf bands to separate further than on a preparative plate of constant thickness. A more uniform developing solvent flow pattern reduces vertical band spreading. All of these factors combine to produce extraordinary resolution in preparative separations on the UNIPLATE-T Taper Plate. 

Analtech Brand Silica Gel G / GF TLC Plates

• Silica Pore: 60Å
• Silica Particle Size: 8-10 μm average
• Binder: Calcium sulfate a.k.a. gypsum (inorganic)
• Hardness: 20-40

Acceptable Solvents:

All typical chromatography solvents (20% water max)

Visualization Techniques:

Compatible with essentially all visualization reagents, evensulfuric acid charring. Spraying reagent is suggested; layer is incompatible with aqueous dipping technique.

General Description:

Considered to be the original “Classic” TLC plate. Gypsum (calcium sulfate) binder produces softer layer for easy scraping and removal of spots or bands for further analysis. This is the best choice when preparative work is needed using thicker coatings to isolate larger quantity of material. Because the binder is inorganic, it is compatible with aggressive visualization techniques such as sulfuric acid charring. Limited water tolerance due to slight solubility of calcium sulfate in water precludes dipping procedure but is applicable with spray techniques.

Silica Gel G & GF UNIPLATES™

Silica Gel G & GF UNIPLATES are the most widely used silica gel TLC plates. No organic substances are added to these products, thus making them compatible with sulfuric acid charring visualization.

These plates are commonly scraped from the glass backing to isolate separated components for other analytical procedures. They are compatible with all organic solvents, however, they are not recommended for use in systems containing greater than 20% water because of the solubility of the gypsum binder in aqueous solutions (For water tolerance see the GHL & GHLF series of plates).

The “G” stands for Gypsum and indicates that calcium sulfate hemihydrate (CaSO4•1/2H2O), a refined form of gypsum, has been included in the adsorbent formulation. Gypsum functions as a binder, improving the cohesion of the adsorbent particles and increasing the adhesion of the layer to the glass substrate. The “F” indicates the presence of a short wave UV indicator that is fluorescent at 254 nm.

Prep-Scored UNIPLATES™

Prep-Scored UNIPLATES are designed especially for preparative TLC procedures where destructive visualization techniques must be used to locate separated zones.

Prep-Scored UNIPLATES are scored 2.5 cm from each vertical edge. After developing, the 2.5 cm strips between each score mark and the edge can be easily “snapped” away from the center portion of the plate.

Visualization of separated zones on these 2.5 cm strips followed by realignment with the center portion provides a convenient method for locating zones to be recovered by scraping.