Miles Scientific Analtech Silica Gel G (Gypsum) Prep TLC Plates, Scored, UNIPLATE-T Taper, Glass-backed, 20 x 20 cm, 25/Pk - P80513

Glass-backed Prep Special TLC Plates

Miles Scientific Analtech Brand Silica Gel G Thin Layer Chromatography (TLC) Plates, UNIPLATE-T Taper Plate, scored - Glass-backed - 20 x 20 cm - 25 plates per box

Product Number: P80513

Weight: 15.10 LBS

• G - (Gypsum) Calcium Sulfate Hemihydrate binder

Tapered Uniplate-T™

The UNIPLATE-T Taper Plate, invented and patented by Analtech, is a unique preparative TLC plate that provides unusually high resolution for a preparative layer. It has a wedge-shaped adsorbent layer, progressing from 300 microns (bottom) to 1700 microns (top). Below the silica layer is a 700-micron-thick preadsorbent zone provided for easier sample application and higher sample resolution (see figure below). Sample concentration prior to separation occurs in this preadsorbent zone.

The tapered adsorbent layer causes low Rf bands to separate further than on a preparative plate of constant thickness. A more uniform developing solvent flow pattern reduces vertical band spreading. All of these factors combine to produce extraordinary resolution in preparative separations on the UNIPLATE-T Taper Plate. 

Analtech Brand Silica Gel G / GF TLC Plates

• Silica Pore: 60Å
• Silica Particle Size: 8-10 μm average
• Binder: Calcium sulfate a.k.a. gypsum (inorganic)
• Hardness: 20-40

Acceptable Solvents:

All typical chromatography solvents (20% water max)

Visualization Techniques:

Compatible with essentially all visualization reagents, evensulfuric acid charring. Spraying reagent is suggested; layer is incompatible with aqueous dipping technique.

General Description:

Considered to be the original “Classic” TLC plate. Gypsum (calcium sulfate) binder produces softer layer for easy scraping and removal of spots or bands for further analysis. This is the best choice when preparative work is needed using thicker coatings to isolate larger quantity of material. Because the binder is inorganic, it is compatible with aggressive visualization techniques such as sulfuric acid charring. Limited water tolerance due to slight solubility of calcium sulfate in water precludes dipping procedure but is applicable with spray techniques.

Silica Gel G & GF UNIPLATES™

Silica Gel G & GF UNIPLATES are the most widely used silica gel TLC plates. No organic substances are added to these products, thus making them compatible with sulfuric acid charring visualization.

These plates are commonly scraped from the glass backing to isolate separated components for other analytical procedures. They are compatible with all organic solvents, however, they are not recommended for use in systems containing greater than 20% water because of the solubility of the gypsum binder in aqueous solutions (For water tolerance see the GHL & GHLF series of plates).

The “G” stands for Gypsum and indicates that calcium sulfate hemihydrate (CaSO4•1/2H2O), a refined form of gypsum, has been included in the adsorbent formulation. Gypsum functions as a binder, improving the cohesion of the adsorbent particles and increasing the adhesion of the layer to the glass substrate. The “F” indicates the presence of a short wave UV indicator that is fluorescent at 254 nm.

Scored UNIPLATES™

Most UNIPLATES can be supplied with the glass backing scored at specific locations to permit convenient “snapping” into smaller plates. This Analtech feature provides increased versatility and can mean cost savings over plates already cut to smaller sizes.

Different adsorbents, mobile phases, and analytical conditions can be tested quickly and economically. A plate can be spotted, then snapped into several smaller plates for developing in different solvent mixtures to determine the best separation.

A plate can be spotted, developed, then snapped into several smaller plates, and then subjected to different visualization techniques.

Scored UNIPLATES are available in two standard formats:

1) 20 x 20 cm scored UNIPLATES have three score marks 5 cm apart. Each plate can be snapped to produce four 5 x 20 cm plates or any multiple of 5 cm width.

2) 10 x 20 cm scored UNIPLATES have seven score marks 2.5 cm apart. Each can be snapped to produce eight 2.5 x 10 cm plates or any multiple of 2.5 cm width.