Nacalai Tesque COSMOSIL HILIC Packed Column, 5 µm, 4.6 mm I.D. x 150 mm - 07056-51

COSMOSIL HILIC is a new column for hydrophilic interaction chromatography with Triazole bonded silica packing material. The hydrophilic interaction chromatography is a variation of normal phase chromatography where a polar stationary phase is used with a mobile phase which contains a high concentration of water miscible organic solvent and a low concentration of aqueous eluent. The main retention mechanism is the partitioning of the polar analytes between the polar stationary and the non-polar mobile phase. As it is also called “aqueous normal phase”, the elution order is similar to that of normal phase and the sample elution is in the order of increasing hydrophilicity. Without using ion-pair reagent COSMOSIL HILIC retains highly polar analytes that would not be retained in reversed phase chromatography. It also shows a weak anion-exchange mechanism with the positively charged stationary phase, thus acidic compound is strongly retained.

Material characteristics