Welcome, Sign In
Email:

Password:

Forgotten Password
Create Account
First time visitor? Create a New Account
Advanced Search
Your cart is empty 		Checkout

Nacalai Tesque COSMOSIL 5HIC Packed Column, 5 µm, 4.6 mm I.D. x 100 mm - 07183-81

Price:
Live chat below or contact us for latest price.


COSMOSIL HIC

COSMOSIL 5HIC is designed for one step desalting and separation of proteins. Hydrophobic interaction chromatography (HIC) is an effective method for purification and separation of proteins (especially enzymes) based on differences in their surface hydrophobicity. Since this method does not use organic solvents like reversed phase chromatography, there is only a little loss in enzyme activity and the tertiary structure of proteins.

Material characteristics

Packing material5HIC
Silica gelhigh purity porous spherical silica
Average particle size5 µm
Average pore sizeapprox. 300Å
Specific surface areaapprox. 150 m2/g
Main interactionhydrophobic interaction

Application data

A buffer with high salt concentration, usually 1-2 mol/l of (NH4)2SO4, is used as an initial mobile phase for adsorption of samples to a weakly hydrophobic stationary phase. The elution is done with a decreasing salt gradient.

Condition
Column size4.6 mm I.D. x 50 mm
Mobile phaseA: 20 mmol/l phosphate buffer + 100 mmol/l Na2SO4 
+ 1.5 mol/l (NH4)2SO4 (pH6.0)
B: 20 mmol/l phosphate buffer + 100 mmol/l Na2SO4 (pH6.7)
B 0 → 100% /10 min linear gradient
Flow rate1.0 ml/min
Temperature30?oC
DetectionUV 220 nm
Sample
  1. Myoglobin 0.5 mg/ml
  2. β-Lactoglobulin 1.0 mg/ml
  3. Hemoglobin 2.5 mg/ml
  4. BSA 1.0 mg/ml
Injection vol.mixture 2.0 μl
Separation of crude β-Glucosidase

Condition
Column size4.6 mm I.D. x 50 mm
Mobile phaseA: 20 mmol/l phosphate buffer + 100 mmol/l Na2SO4 
+ 1.5 mol/l (NH4)2SO4 (pH6.0)
B: 20 mmol/l phosphate buffer + 100 mmol/l Na2SO4 (pH6.7)
B 0 → 100% /10 min linear gradient
Flow rate1.0 ml/min
Temperature30?oC
DetectionUV 220 nm
SampleCrude β-Glulosidase 10.0 mg/ml
Injection vol.1.5 μl

  • Model: 07183-81
  • Manufactured by: Nacalai Tesque

Product 41/68

Previous
Return to the Product List
Next
Desktop Version
© 2025 UVISON Technologies Limited. All rights reserved  |  Registered in England and Wales under company number 4718736 and VAT number GB 702 1041 10
wabusiness template