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Purospher® RP-18 250-4 LiChroCART® HPLC Cartridge, 90 Å, 5 μm, 4 x 250 mm - 150144

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 Purospher® RP-18

Inner Diameter (I.D.): 4 mm
Length: 250 mm
Package Contents: 1 Cartridge


Purospher® RP-18 is designed for peak-tailing free chromatography of strongly basic compounds with simple, neutral 
mobile phases. Thus, method development time and consequently analyses costs are reduced. Additionally, Purospher® 
RP-18 allows the separation of hydrophilic compounds using up to 100% aqueous eluents.

The key for understanding the properties of Purospher® RP-18 lies in the chemistry of the base, high purity silica with 
virtually no metal contaminants present. In addition, multi-step chemical modification and deactivation by polymeric 
coating and amino shielding of the surface eliminate unpredictable interactions from residual silanols. This results in 
symmetric peak shapes of basic and chelating analytes without the addition of any modifiers to the mobile phase. 
Due to the amino endcapping step, Purospher® RP-18 is not suitable for the separation of acidic compounds. The high 
chemical stability of Purospher® RP-18 permits the usage of mobile phase conditions at pH 8 for a long period of 
time without decline of performance.

Purospher® RP-18 benefits
  • Symmetrical peaks for basic, chelating, and polar analytes
  • Fast method development for basic compounds
With LiChroCART® cartridges the user works with re-usable endfittings which fit different cartridge lengths. 
the cartridges may be changed within the shortest possible time. Changing a separation cartridge from a length 
of 125 to 250 mm and back presents no problems. Furthermore, the adaptation of internal diameter (3 mm or 2 mm) 
to the analysis problem is possible within a few minutes. The endfittings are designed to allow the cartridges to be 
hand-sealed at normal working pressures of 150 to 200 bar without the need for any tools. Only at higher pressures 
may further tightening with a wrench become necessary.


Specifications of Purospher® RP-18

Sorbent characteristics: High-purity silica particles with C18 modification and deactivation of surface; amino shielding not suitable for acidic compounds!
Particle shape spherical
Particle size: 5 μm
Pore size: 9 nm (90 Å)
Pore volume: 1.05 mL/g
Specific surface area: 480 m2/g
Carbon load: 17% C
Efficiency: 80,000 N/m
pH range: pH 2 - 8
Shipping eluent: Acetonitrile/Water

  • Model: 1.50144.0001
  • Manufactured by: Merck Chemicals