Shimadzu Ion Exchange Columns

Shim-pack™ Ion Exchange Chromatography Columns

Ion exchange chromatography (IEX) is a separation method using the difference of charge states between the mobile phase and analytes from the stationary phase in the column. It is mainly used for the analysis of ionic compounds. There are two types of IEX; anion exchange chromatography and cation exchange chromatography, and the type used depends on the ionic strength of theTable 1. Typical Ion Exchange Functional Groups Used In the Stationary Phase ion exchange group. Table 1 shows typical functional groups per IEX stationary phase type. Strong ion-exchange functional groups can always be ionized, while ionization conditions of weak ion-exchange groups can vary depending on the pH of the mobile phase. The appropriate stationary phase is selected according to the charge and properties of the analyte.

Note that when ion exchange chromatography is applied, cations and anions cannot be separated within a single analysis.