Aqueous SEC (GFC) Columns: Silica-based
PROTEIN KW columns separation by size also for high molecular weight
The silica-based SEC (GFC) column for proteins and enzymes
PROTEIN KW402.5-4F | 4.6 x 300 mm High performance semi-micro column
Shodex Product Number: F6989201
Specifications
Description | PROTEIN KW402.5-4F 300x4.6mm |
---|---|
Chemistry Producers Term | KW |
Base Material | Silica |
Mode of Separation | Aqueous SEC Gel Filtration (GFC) |
Functional group | Hydroxyl |
Plates per column | ≥ 35,000 |
Housing material | steel |
Maximum pressure | 10 MPa (100 bar) |
Usual flow rate | 0.35 mL/min |
Maximum flow rate | 0.5 mL/min |
Temperature range | 5 to 45°C |
pH range | 3.0 to 7.5 |
Salt concentration | ≤ 0.3 M |
Shipping solvent | H2O |
MW range | 5,000 - 70,000 |
Exclusion limit | 150,000 |
Internal Diameter | 4.6 mm |
Length | 300 mm |
Brand | PROTEIN |
Particle Morphology | spherical, porous |
Particle Size | 3 µm |
pH Minimum | 3.0 |
pH Maximum | 7.5 |
Pore Size | 400 Å |
USP Class | L20, L33, L59 |
PROTEIN KW400 series
High-performance semi-micro column version of the PROTEIN KW-800 series. The theoretical plate number and the sensitivity are improved by reducing the diameter of the packed particle to 3 micrometers.
- Silica-based packed columns for aqueous SEC (GFC) analysis
- Suitable for the analysis of proteins and enzymes
- Reduced packing material particle size enhances column performance
- Three to four-fold higher sensitivity than KW-800 series
- KW405-4F is applicable analyzing samples with molecular weight above 1,000,000
- USP L20 - Dihydroxypropane groups chemically bonded to porous silica or hybrid particles, 1.5-10 μm in diameter, or a monolithic silica rod.
- USP L33 - Packing having the capacity to separate dextrans by molecular size over a range of 4,000 to 500,000 Da. It is spherical, silica-based, and processed to provide pH stability.
- USP L59 - Packing for the size-exclusion separations of proteins (separation by molecular weight) over the range of 5 to 7000 kDa. The packing is a spherical 1.5- to 10-μm, silica or hybrid packing with a hydrophilic coating.
- Suitable for analysis of Polymers - Biological macromolecules (Proteins, Peptides, Nucleic acids, etc.)
High performance semi-micro columns | Base Material: Silica | Usable pH Range: pH3.0 - 7.5
KW400 series is recommended to be used with semi-micro type devices
Product Code | Product Name | * Plate Number (TP/column) |
Particle Size (μm) |
Pore Size (Å) |
Column Size (mm) I.D. x Length |
Shipping Solvent |
F6989201 | KW402.5-4F | ≥ 35,000 | 3 | 400 | 4.6 x 300 | H2O |
F6989202 | KW403-4F | ≥ 35,000 | 3 | 800 | 4.6 x 300 | H2O |
F6989203 | KW404-4F | ≥ 25,000 | 5 | 1,500 | 4.6 x 300 | H2O |
F6989204 | KW405-4F | ≥ 25,000 | 5 | 2,000 | 4.6 x 300 | H2O |
F6700132 | KW400G-4A | (guard column) | 5 | — | 4.6 x 10 | H2O |
* Measured with uridine
- Model: F6989201
- Manufactured by: Showa Denko