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Shodex HPLC Column PROTEIN KW402.5-4F ID 4,6mm L 300mm - F6989201

Price: £1,431.83

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Aqueous SEC (GFC) Columns: Silica-based

PROTEIN KW columns separation by size also for high molecular weight
The silica-based SEC (GFC) column for proteins and enzymes

PROTEIN KW402.5-4F | 4.6 x 300 mm High performance semi-micro column

Shodex Product Number: F6989201


Description PROTEIN KW402.5-4F 300x4.6mm
Chemistry Producers Term KW
Base Material Silica
Mode of Separation Aqueous SEC Gel Filtration (GFC)
Functional group Hydroxyl
Plates per column ≥ 35,000
Housing material steel
Maximum pressure 10 MPa (100 bar)
Usual flow rate 0.35 mL/min
Maximum flow rate 0.5 mL/min
Temperature range 5 to 45°C
pH range 3.0 to 7.5
Salt concentration  ≤ 0.3 M
Shipping solvent H2O
MW range 5,000 - 70,000
Exclusion limit 150,000
Internal Diameter 4.6 mm
Length 300 mm
Particle Morphology spherical, porous
Particle Size 3 µm
pH Minimum 3.0
pH Maximum 7.5
Pore Size 400 Å
USP Class L20, L33, L59

PROTEIN KW400 series
High-performance semi-micro column version of the PROTEIN KW-800 series. The theoretical plate number and the sensitivity are improved by reducing the diameter of the packed particle to 3 micrometers. 

  • Silica-based packed columns for aqueous SEC (GFC) analysis
  • Suitable for the analysis of proteins and enzymes
  • Reduced packing material particle size enhances column performance
  • Three to four-fold higher sensitivity than KW-800 series
  • KW405-4F is applicable analyzing samples with molecular weight above 1,000,000
  • USP L20 - Dihydroxypropane groups chemically bonded to porous silica or hybrid particles, 1.5-10 μm in diameter, or a monolithic silica rod.
  • USP L33 - Packing having the capacity to separate dextrans by molecular size over a range of 4,000 to 500,000 Da. It is spherical, silica-based, and processed to provide pH stability.
  • USP L59 - Packing for the size-exclusion separations of proteins (separation by molecular weight) over the range of 5 to 7000 kDa. The packing is a spherical 1.5- to 10-μm, silica or hybrid packing with a hydrophilic coating.
  • Suitable for analysis of Polymers - Biological macromolecules (Proteins, Peptides, Nucleic acids, etc.)

High performance semi-micro columns | Base Material: Silica | Usable pH Range: pH3.0 - 7.5
KW400 series is recommended to be used with semi-micro type devices

Product Code Product Name * Plate Number
Particle Size
Pore Size
Column Size (mm)
I.D. x Length
Shipping Solvent
F6989201 KW402.5-4F ≥ 35,000 3 400 4.6 x 300 H2O
F6989202 KW403-4F ≥ 35,000 3 800 4.6 x 300 H2O
F6989203 KW404-4F ≥ 25,000 5 1,500 4.6 x 300 H2O
F6989204 KW405-4F ≥ 25,000 5 2,000 4.6 x 300 H2O
F6700132 KW400G-4A (guard column) 5 4.6 x 10 H2O

* Measured with uridine


  • Model: F6989201
  • Manufactured by: Showa Denko